Abstract
Juniperus communis L. (= J. communis var. communis ) and Juniperus nana Willd. (= J. communis var. saxatilis ) are subspecies of juniper. J. communis grows widely in both hemispheres, primarily in lower elevations while J. nana is mainly observed in high mountains. Although they can be distinguished by morphological features, it is not known whether they are genetically and phytochemically distinct entities. We aimed to check whether it is possible to distinguish these two plants (i) by pharmaceutically important chemical traits and (ii) on the basis of intraspecifically highly polymorphic fragment of chloroplast DNA. We used GC with achiral as well as with enantioselective stationary phase columns to identify the main monoterpenes of the essential oil. Sequence analysis of the trnL (UAA)-trnF (GAA) intergenic spacer of the chloroplast genome was used as a genetic marker of taxonomic identity between these two subspecies. The chromatographic analysis showed the existence of three chemical races - the α-pinene type, the sabinene type and one with intermediate contents of these terpenes among both J. communis and J. nana . Surprisingly, sequence analysis of trnL (UAA)-trnF (GAA) revealed 100 % similarity between the common and the dwarf juniper. Thus, the monoterpene pattern is related to geographical origin, and not to the species identity. We suggest that the three chemical races identified in the present study should be considered as separate sources of pharmaceutical raw material. Our results demonstrate that the contents of α-pinene and sabinene may be applied as a quick diagnostic test for preeliminary evaluation of plant material.
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