Planta Med 2006; 72 - P_065
DOI: 10.1055/s-2006-949865

Inhibitory Effects of the Constituents of Prunus mume on Bradykinin and Prostaglandin E2 Production in Abdominal Cavities of Mice

H Ina 1, K Yamada 1, K Matsumoto 2, T Miyazaki 1
  • 1School of Pharmacy, Tokyo University of Pharmacy & Life Science, 1432–1 Horinouchi, Hachioji, Tokyo 192–0392, Japan
  • 2Umekenkyuukai Foundation, 1–1-26 Gakuenchou, Settsu, Osaka 566–8566, Japan

The fruits of Prunus mume Sieb. et Zucc. (Rosaceae) have been traditionally used as medicinal food in Japan. In regard to the chemical constituents, we previously reported on isolation of benzyl β-D-glucopyranoside (BG) and chlorogenic acid (CA) [1].

In the course of our research on the pharmacologically active constituents of the fruits of P. mume, we recently found that BG and CA inhibited acetic acid-induced writhing behavior of mice. To elucidate the analgesic mechanisms of BG and CA, we compared the inhibitory effects of BG and CA on bradykinin and prostaglandin E2 production [2, 3] in abdominal cavities of mice with those of aspirin (Asp). In this symposium we report the effects of BG, CA and ASP on acetic acid-induced writhing behavior of mice, and on bradykinin and prostaglandin E2 production in abdominal cavities of mice.

BG, CA and Asp equally inhibited bradykinin production. Though the inhibitory effects of BG and CA were smaller than that of Asp in the case of prostaglandin E2 production, BG and CA meaningfully inhibited it. These results indicate that the inhibitory effects of BG and CA on acetic acid-induced writhing behavior were brought about by inhibiting both bradykinin and prostagrandin E2 production in abdominal cavities of mice.

References: 1. Ina, H. et al. (1999), Nat. Med. 53: 109–110. 2. Ando, T. et al. (1982), Recent Progress on Kinins, ed. by Fritz, H., Dietze, G. et al., Birkhauser Verlag, Stuttgart, pp. 222–232. 3. Kawano, K. (1987), Enshou, 7: 511–515.