Analysis of the Physiological Activity from Kalopanax septemlobus Koidz. Extracts in Korea

Aiming to find new uses and to select clones that contain high physiological active materials, stem and root barks of Kalopanax septemlobus Koidz., were analyzed to measure cell cytotoxicity(NR assay, MTT assay), anti-lipid peroxidation(TBA method), anti-free radical activity(DPPH test, NBT test) and oxidative stress(DCFH-DA method).

NR50 and MTT50 values were 0.002˜0.33mg/mL, 0.003˜0.80mg/mL, respectively, and MeOH treatment of stem barks showed lowest toxicity. TBA analysis showed many differences by extraction methods and sampling parts. MeOH treatment of stem barks showed the best result, 98% and that was better than control, vitamin C. Extracts of Kalopanax septemlobus showed superior anti-free radical activity on the DPPH and NBT tests. MeOH 0.1mg/mL treatment of stem bark showed the best, 96% on the DPPH test and that was better than control, vitamin E. Also, hot-water extracts soluble treatment of stem barks showed the best, 95%. Hot-water extracts insoluble treatment of root barks showed the best in DCFH-DA analysis.

The stem bark of Kalopanax septemlobus has been used in traditional Korean medicine for anti-inflammatory, expectorant, tranquilizer and effective on anti-rheumatic.

Thirteen kinds of chemical components were isolated from the extracts of the dried stem bark of K. septemlobus. On the basis of physico-chemical, spectroscopic data and in comparison with those of authentic samples or values reported in the literatures, they were identified as β-sitosterol, oleanolic acid, caffeic acid, kalopanaxsaponin A, chlorogenic acid, protocatechuic acid, 3, 3'-bis(3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran), (-)-balanophonin, liriodendrin, syringin, kalopanaxsaponin B, kalopanaxsaponin I and kalopanxsaponin H, respectively.