Planta Med 2006; 72 - P_153
DOI: 10.1055/s-2006-949953

Quantitative Determination of 1-Deoxynojirimycin in Mulberry Leaves using Liquid Chromatography-Tandem Mass Spectrometry

N Nuengchamnong 1, K Ingkaninan 2, W Kaewruang 3, S Wongareonwanakij 3, B Hongthongdaeng 3
  • 1Regional Medical Sciences Center Phitsanulok, Department of Medical Sciences, Ministry of Public Health 65000 Thailand. nitra@dmsc.moph.go.th
  • 2Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University 65000 Thailand
  • 3The Queen Sirikit Institute of Sericulture, Chatuchak, Bangkok 10900, Thailand

An HPLC-MS/MS method was developed for the quantitative determination of 1-deoxynojirimycin (DNJ), a potent glucosidase inhibitor presented in mulberry leaves (Morus alba L.). DNJ was separated from an extract of mulberry leaves on a TSK gel Amide-80 column using a mixture of 0.1% formic acid and acetonitrile as the mobile phase at a flow rate of 0.6 mL/min. A triple quardupole mass spectrometry using turbo spray ionization source in the positive ion mode under multiple reaction monitoring with the [M-H]+ ions, m/z164.4/ 109.9 was used. The detection limit (S/N=3) was 75 pg and quantification limit (S/N=10) was 100 pg. The DNJ was higher in young shoots of mulberry in comparison with young leaves and mature leaves.