A Novel Isoflavonoid from Millettia puguensis

Abstract

From the roots of Millettia puguensis (Leguminosae), a novel isoflavonoid (5), 2′-methoxy-4′,5′-methylenedioxy-7,8-[2-(1-methylethenyl)furo]isoflavone, and four known compounds, i. e., lupeol (1), (-)-maackiain (2), 6,7-dimethoxy-3′,4′-methylenedioxyisoflavone (3) and 7,2′-dimethoxy-4′,5′-methylenedioxyisoflavone (4) were isolated and identified by ¹H-, ¹³C-NMR and mass spectroscopy. All compounds were evaluated for their antiprotozoal and cytotoxic activities, but only a moderate antileishmanial activity was observed for compound 3 (IC₅₀ = 32 μM against Leishmania infantum), and a moderate cytotoxicity for compound 2 (IC₅₀ = 43 μM on MRC-5 cells).

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