Mass Production of Ginseng (Panax ginseng) Embryoids on Media Containing High Concentrations of Sugar1

Isao Asaka; Ichio Ii; Masao Hirotani; Yoshihisa Asada; Takafumi Yoshokawa; Tsutomu Furuya

doi:10.1055/s-2006-959438

Planta Medica, Table of Contents

Planta Med 1994; 60(2): 146-148
DOI: 10.1055/s-2006-959438

Papers

Mass Production of Ginseng (Panax ginseng) Embryoids on Media Containing High Concentrations of Sugar¹

Isao Asaka² , Ichio Ii² , Masao Hirotani³ , Yoshihisa Asada³ , Takafumi Yoshokawa³ , Tsutomu Furuya³

²Life Science Center, Iwaki Glass Co. Ltd., Gyoda 1-50-1, Funabashi-shi, Chiba 273, Japan
³School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108, Japan

1 Part 87 in the series “Studies on Plant Tissue Culture”. For Part 86 see Ref.(1)

Abstract

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Abstract

A lot of ginseng embryoids were produced by culturing on high concentrations of sugar media from the embryogenic tissues obtained by moderately high temperature treatment. When the sucrose concentration was 100 g/l, the number of embryoids produced were over 100 pieces per g of inoculum weight. It was about ten times of that produced by culturing on 30 g/l of sucrose. Glucose showed an effect similar to sucrose on the basis of weight percentage. However, mannitol did not show this effect. The embryoids obtained by these processes redifferentiated to normal plantlets on culturing on the medium containing 30 g/l of sucrose. The saponin components of the tissue containing embryoids showed a similar pattern to those of natural ginseng by HPLC.

Key words

Panax ginseng - Araliaceae - tissue culture - embryoid - propagation - sugar

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