Perfusion with Picroliv Reverses Biochemical Changes Induced in Livers of Rats Toxicated with Galactosamine or Thioacetamide¹

Abstract

Perfusion of liver of rats toxicated with galactosamine or thioacetamide with a 0.02% solution of picroliv (glycoside fraction of Picrorhiza kurroa) for 30 min (1 ml/min; 6 mg/rat), significantly reversed toxicant-induced changes in the activities of several enzymes. Galactosamine induced increases in the activities of alkaline phosphatase, γ-glutamyl transpeptidase, acid ribonuclease, acid phosphatase, succinate dehydrogenase and decreases in the activities of Na⁺-K⁺-adenosine triphosphatase (ATPase) and glucose-6-phosphatase (reversed by 40-87%). Similarly, thioacetamide-induced inhibitions of the activities of Na⁺-K⁺-ATPase, Ca^{+ +}-ATPase, Mg^{+ +}-ATPase, succinate dehydrogenase and elevations in the activities of alkaline phosphatase, γ-glutamyl transpeptidase, and acid ribonuclease were also significantly reversed. A significant reversal of the toxicants-induced decrease in [¹⁴ C]-leucine incorporation was also observed. These results indicate that picroliv can also reverse D-galactosamine-or thioacetamide-induced hepatic damage in rats.

Key words

Galactosamine - thioacetamide - picroliv perfusion - hepatotoxicity - hepatic enzymes