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Planta Med 1993; 59(5): 432-435
DOI: 10.1055/s-2006-959726
Paper

© Georg Thieme Verlag Stuttgart · New York

Tropane Alkaloid Levels in the Leaves of Micropropagated Datura innoxia Plants

E. Gontier1 , 2 , M. A. Fliniaux3 , J. N. Barbotin2 , B. S. Sangwan-Norrel1
  • 1Laboratoire Androgenèso et Biotechnologie, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue St. Leu, F-80039 Amiens Cedex, France
  • 2Laboratoire de Génie cellulaire Faculté des Sciences, Université de Picardie Jules Verne, 33 rue St. Leu, F-80039 Amiens Cedex, France
  • 3Laboratoire de Pharmacognosie, UFR de Pharmacie, Université de Picardie Jules Verne, 3 place Dewailly, F-80000 Amiens, France
Further Information

Publication History

1992

1992

Publication Date:
04 January 2007 (online)

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Abstract

Three androgenic Datura innoxia Mill. (Solanaceae) plants which had been characterised in regard to their tropane alkaloid levels were micropropagated through axillary buds. The alkaloid contents in the leaves of more than 300 micropropagated plants were investigated by ELISA and HPLC, after one and two culture cycles and compared to those of one of seedlings obtained from the same original line from which the androgenic plants had been produced. The plant with the higher alkaloid level yielded the population with the most alkaloids. After a second cycle of micropropagation, all the populations contained more alkaloids than those they had been derived from. All the micropropagated plant populations contained higher levels of alkaloids than plants grown from seeds.

Key words

Datura innoxia - micropropagation - tropane alkaloids

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