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Planta Med 1992; 58(1): 31-34
DOI: 10.1055/s-2006-961384
Paper

© Georg Thieme Verlag Stuttgart · New York

Transformation of Progesterone by Traemetes hispida

J. S. Dahiya1 , 2
  • 1Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada
  • 2ABI Biotechnology Incorporated, 40 Scurfield Blvd., Winnipeg, Manitoba R3Y 1G4, Canada
Further Information

Publication History

1991

Publication Date:
04 January 2007 (online)

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Abstract

Traemetes hispida was found to convert progesterone into a mixture of various products. Five products were isolated in sufficient amounts by HPLC analysis for structural identification and characterization. Three products were found to have two hydroxy moieties on axial and equatorial positions at 7β,15β, 6β, 11α, and 11α, 15β sites. The other two products had an extra 17α-hydroxy on the above dihydroxy derivatives to give trihydroxyprogesterone. The hydroxylating steps caused by this strain were greatly influenced by the 17β side chain, especially by the C-20 moiety in the steroidal skeleton.

Key words

Traemetes hispida - fungus - progesterone - transformation - 1H-NMR - 13C-NMR - mass spectral analysis