Abstract
The main furanocoumarins from the roots of Peucedanum palustre were separated with a RP-HPLC method. The mobile phase for the separation of the
eight main compounds (oxypeucedanin hydrate, bergapten, oxypeucedanin, isobyakangelicin
angelate, ostruthol, imperatorin, isoimperatorin, and columbianadin) was optimized
with the “PRISMA” model, which is a multi-solvent optimization system. The chromatographic
method can be applied for quantitative determination, as was demonstrated for different
samples of P. palustre. The purity and identity of the peaks were controlled by diode-array detection and
comparison with standards.
