Isolation and High Performance Liquid Chromatography (HPLC) of Isoflavonoids from the Pueraria Root

Yukio Ohshima; Toru Okuyama; Kunio Takahashi; Toshio Takizawa; Shoji Shibata

doi:10.1055/s-2006-962420

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Planta Med 1988; 54(3): 250-254
DOI: 10.1055/s-2006-962420

Papers

Isolation and High Performance Liquid Chromatography (HPLC) of Isoflavonoids from the Pueraria Root

Yukio Ohshima¹ , Toru Okuyama¹ , Kunio Takahashi¹ , Toshio Takizawa² , Shoji Shibata¹ ^, ²

¹Meiji College of Pharmacy, Nozawa 1-35-23, Setagaya-ku, Tokyo 154, Japan.
²Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo. 113, Japan.

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Publication History

1987

Publication Date:
24 January 2007 (online)

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Abstract

From a Chinese drug Gegen, the root of Pueraria lobata (Willd.) Ohwi or P. pseudohirsuta Tang et Wang (Leguminosae), several isoflavonoid compounds were isolated. Besides the known compounds, puerarin (1), daidzin (2), daidzein (3), and formononetin (4), the presence of pueraria glycosides (PG) 1-6 and puerarol (9) in the Pueraria root extracts was revealed by TLC and HPLC. The chemical structures of PG-1, 2, 3, and 6 (5-8) as well as puerarol (9) were supported by their spectral data.