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Planta Med 1987; 53(2): 148-153
DOI: 10.1055/s-2006-962658
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© Georg Thieme Verlag Stuttgart · New York

Effects of Caffeoylglycosides on Arachidonate Metabolism in Leukocytes

Yoshiyuki Kimura1 , Hiromichi Okuda1 , Sansei Nishibe2 , Shigeru Arichi3
  • 12nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02, Japan.
  • 2Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University, Ishikari-Tobetsu, Hokkaido 061-02, Japan.
  • 3The Research Institute of Oriental Medicine, Kinki University, Sayama-cho, Minamikawachi-gun, Osaka 589, Japan.
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Publikationsverlauf

1986

Publikationsdatum:
24. Januar 2007 (online)

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Abstract

The effects of caffeoylglycosides (forsythiaside, suspensaside, acteoside, and β-hydroxyacteoside), isolated from dried fruits of Forsythia species, on the formations of the 5-lipoxygenase products 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4), the 15-lipoxygenase product 15-hydroxy-5,8,11,13-eicosastetraenoic acid (15-HETE) and the cyclooxygenase products 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), thromboxane B2 and 6-keto-PGF1α in rat peritoneal cells and human peripheral polymorphonuclear leukocytes were studied. Forsythiaside, suspensaside, acteoside, and β-hydroxyacteoside were found to inhibit selectively the formation of the 5-lipoxygenase products 5-HETE and LTB4 in human peripheral polymorphonuclear leukocytes; their concentrations for 50% inhibition (IC50) of 5-HETE formation being 1.92, 49.0, 4.85, and 40.0 µM, and their IC50 values for LTB4 formation being 1.01, 8.85, 2.93, and 8.50 µM, respectively.

Forsythiaside, suspensaside, acteoside, and β-hydroxyacteoside inhibited the formation of 5-HETE from arachidonic acid in rat peritoneal cells, their IC50 values on its formation being 2.50, 7.97, 5.27, and 19.3 µM, respectively. Forsythiaside caused non-competitive inhibition of 5-HETE formation.