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Planta Med 1987; 53(2): 189-192
DOI: 10.1055/s-2006-962669
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© Georg Thieme Verlag Stuttgart · New York

HPLC-Analyse der Flavonoidglykoside aus Betulae folium1

HPLC Analysis of the Flavonoid Glycosides from Betulae foliumKarin Dallenbach-Tölke2 , Szabolcs Nyirediy2 , 3 , Beat Meier2 , Otto Sticher2
  • 2Pharmazeutisches Institut, Eidgenössische Technische Hochschule Zürich, CH-8092 Zürich, Schweiz.
  • 3Pharm. Institut der ETH Zürich, von der Medizinischen Universität Semmelweis, Institut für Pharmakognosie, H-1085 Budapest, Ungarn.
1 Teil eines Vortrages anläßlich der 33. Vortragstagung der Gesellschaft für Arzneipflanzenforschung, 22.-27. IX. 1985, Regensburg, BRD (vgl. 9).
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1986

Publication Date:
24 January 2007 (online)

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Abstract

The main flavonoid glycosides from Betulae folium are separated with a RP-HPLC method. The mobile phase for the separation of the seven most important compounds (rutin, hyperin, quercetin-3-arabinofuranoside, quercetin-3-arabinopyranoside, quercetin-3-rhamnoside, quercetin-3-glucuronide, and myricetin-3-galactoside) was optimized with the “PRISMA” model, which is a multisolvent optimization system. The isocratic separations were finished within 30 minutes and the peak purity was controlled with diode-array detection. The method can be applied for quantitative determination, as was demonstrated for different samples of B. pendula and B. pubescens.