Planta Med 1985; 51(5): 364-367
DOI: 10.1055/s-2007-969521
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Separation and Quantitation of Protoanemonin in Ranunculaceae by Normal- and Reversed-Phase HPLC

A. Bonora, G. Dall'Olio, A. Bruni
  • Institute of Botany, University of Ferrara, Corso Porta Mare 2, I-44100 Ferrara, Italy
Further Information

Publication History

1984

1985

Publication Date:
26 February 2007 (online)

Abstract

A method is described for the extraction and high-performance liquid chromatographic separation and quantitation of the lactone protoanemonin in leaves of Ranunculaceae. For samples with a protoanemonin concentration over 10 µg/g W. W., a reversed-phase technique using a Lichrosorb RP 18 column and a binary solvent system was developed. Alternatively, for samples with a lower lactone concentration, a normal-phase technique with a Lichrosorb Si 60 and quaternary elution system was elaborated. Protoanemonin was detected at 258 nm; its calibration curves were established, and its response factor was calculated using, as standard, the pure compound extracted from Helleborus niger. A survey of ten different taxa of Ranunculaceae was performed and it showed the suitability of the method for routine work with high sensitivity limit.

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