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Planta Med 1984; 50(3): 235-238
DOI: 10.1055/s-2007-969686
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Radioimmunoassay for the Quantitative Determination of Quinine in Cultured Plant Tissues

R. J. Robins, A. J. Webb, M. J. C. Rhodes, J. Payne, M. R. A. Morgan
  • Agricultural and Food Research Council, Food Research Institute, Colney Lane, Norwich NR4 7UA, U.K.
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Publication History

Publication Date:
26 February 2007 (online)

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Abstract

The development of a radioimmunossay for the determination of picogram amounts of quinine is described. The antiserum, raised against a conjugate of quinine and bovine serum albumin, is highly specific to the Cinchona quinoline alkaloids having the 8S configuration and will not cross-react to any detectable extent with the 8R series. Neither will it cross-react with quininone or cinchonidinone, the common 9-keto precursors. Using this assay a number of clones of C. ledgeriana have been screened for their quinine content.

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