Conversion of Elymoclavine to Paspalic Acid by a Particulate Fraction from an Ergotamine-Producing Strain of Claviceps sp.

S.-U. Kim; Y.-J. Cho; H. G. Floss; J. A. Anderson

doi:10.1055/s-2007-969910

Planta Medica, Inhaltsverzeichnis

Planta Med 1983; 48(7): 145-148
DOI: 10.1055/s-2007-969910

Research Articles

Conversion of Elymoclavine to Paspalic Acid by a Particulate Fraction from an Ergotamine-Producing Strain of Claviceps sp.

S.-U. Kim¹ , Y.-J. Cho¹ , H. G. Floss² , J. A. Anderson¹

¹Department of Chemistry, Texas Tech University, Lubbock, TX 79409
²Department of Chemistry, Ohio State University, Columbus, Ohio 43210

Abstract

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Abstract

The particulate fraction from the ergotamine-producing strain Claviceps sp. PCCE1 catalyzed the conversion of [¹⁴C]elymoclavine to paspalic acid. NADPH was required. Maximum conversion was 95%. Carbon monoxide (CO:0₂, 4:1) and SK&F 525A (1.0 mM), cytochrome P-450 inhibitors, inhibited the conversion 94% and 50%, respectively. Minor amounts of paspalic acid (0.1 mg/1) were present in cultures. The particulate fraction from Claviceps sp. SD 58, which accumulates elymoclavine in cultures, lacked activity for the conversion of elymoclavine to paspalic acid.

Key Word Index

Claviceps sp. - Clavicipitaceae - Elymoclavine - Paspalic acid - Cell-free

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