Abstract
Rapid and simple regeneration of Pinellia ternata has been achieved by tissue culture. The addition of 2,4-dichlorophenoxy acetic acid
(2,4-D; 0.5 ppm) and kinetin (1 ppm) stimulated production of a maximum number of
regenerated plantlets, while callus formation was induced by concentrations of 2,4-D
higher than 1 ppm. α-Naphthaleneacetic acid (NAA) was also favourable for regeneration
but was less effective than 2,4-D. Growth with indole-3-acetic acid (IAA) and kinetin
favoured callus formation in general, but the rate of callus growth was extremely
slow.
These regenerated plantlets flowered true to their parental strains when transferred
to soil and cultivated for 4.5 months.
Key Word Index
Pinellia ternata - Araceae - Regeneration - Tissue Culture - Phytohormone Control
