Planta Med 1981; 42(6): 160-166
DOI: 10.1055/s-2007-971620
Research Articles

© Hippokrates Verlag Stuttgart

Biotransformation of Cardenolides by Plant Cell Cultures1

II. Metabolism of Gitoxigenin and its Derivatives by Suspension Cultures of Daucus carota A. Jones, I. A. Veliky
  • Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada K1A OR6
1 NRCC No. 19260.
Further Information

Publication History

Publication Date:
30 March 2007 (online)

Abstract

A culture of Daucus carota cell line Ca68, previously shown to specifically hydroxylate digitoxigenin at the C-5 position, has been shown also to convert gitoxigenin and oleandrigenin to their corresponding 5β-hydroxy derivatives. These two products have not been identified previously. 5β-Hydroxygitoxigenin has been identified by physico-chemical methods and data on its mass spectrum and 13C-NMR spectrum are reported. 5β-Hydroxyoleandrigenin was identified from its high resolution mass spectrum. Addition of gitoxigenin at 72 hrs after inoculation resulted in a two fold increase in the rate of bioconversion compared to addition at 0 hr. The results are discussed in relation to other biotransformation reactions and with respect to improving the yields of plant products and the formation of novel compounds.

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