Anti-Inflammatory Ergostanes from the Basidiomata of Antrodia salmonea

Abstract

Three new anti-oxidative ergostanes, methyl antcinate L (1), antcin M (2), and methyl antcinate K (3), together with nine additional known compounds, 3-ketodehydrosulphurenic acid, sulphurenic acid, dehydrosulphurenic acid, 3β,15α-dihydroxylanosta-7,9(11),24-trien-21-oic acid, zhankuic acid A, zhankuic acid B, zhankuic acid C, antcin C, and antcin K were isolated from the basidiomata of Antrodia salmonea, a newly identified species of Antrodia (Polyporaceae) in Taiwan. These three new compounds were identified as methyl 3α,7α,12α-trihydroxy-4α-methylergosta-8,24(29)-dien-11-on-26-oate (1), 3α,12α-dihydroxy-4α-methylergosta-8,24(29)-dien-11-on-26-oic acid (2), and methyl 3α,4β,7β-trihydroxy-4α-methylergosta-8,24(29)-dien-11-on-26-oate (3) by spectroscopic analysis. We studied their antioxidative potential on the production of reactive oxygen species and nitric oxide (NO) in neutrophils and microglial cells, respectively. Compounds 1 - 3 displayed potent antioxidative activity with IC₅₀ values of around 2.0 - 8.8 μM that was partially due to inhibition (6 - 67 %) of NADPH oxidase activity but not through direct radical-scavenging properties. Compounds 1 - 3 also inhibited NO production with IC₅₀ values of around 1.7 - 16.5 μM and were more potent than a non-specific NOS inhibitor. We conclude that these three new compounds 1, 2, and 3 exhibit anti-inflammatory activities in activated inflammatory cells.

Key words

Polyporaceae - Antrodia salmonea - ergostane - lanostane - microglial cell - neutrophils - reactive oxygen species - nitric oxide (NO)

References

1 Y. H. Wang and T. T. Chang contributed to this work equally

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