Planta Med 2007; 73 - P_189
DOI: 10.1055/s-2007-986970

Antimicrobial studies on Origanum acutidens (Hand.-Mazz.) Ietswaart

N Yiğit 1, D Yiğit 2, U Özgen 3, E Sezen 3, HG Sevindik 3, AE Aktaş 4
  • 1Atatürk University, Health Services Vocational Training School, Medical Laboratory Program, Erzurum, 25070, Turkey
  • 2Erzincan University, Faculty of Education, Erzincan, 24030, Turkey
  • 3Atatürk University, Faculty of Pharmacy, Department of Pharmacognosy, Erzurum, 25240, Turkey
  • 4Atatürk University, Faculty of Medicine, Microbiology and Clinical Microbiology Department, Erzurum, 25240, Turkey

Many Origanum species (Lamiaceae) known as „Kekik, kekik otu, keklik otu, mercanköşk“ are used by the public especially as a spice and for its several medicinal effects in Turkey [1]. O. acutidens is an endemic species and grows mainly in Central and East Anatolia [2]. Ursolic and oleanolic acid; rosmarinic and lithospermic acid; vicenin-2, betulalbuside A, 8-OH-linaloyl glucoside were isolated from aerial parts of O. acutidens [3]. In this study, the antibacterial and anticandidal activity of O. acutidens methanol extract and fractions (n-hexane, CHCl3, EtOAc, aqueous) prepared from methanol extract were evaluated against clinical bacterial and candida strains. Antimicrobial test was then carried out by disc-diffusion method using suspension containing 108 CFU/ml of bacteria, 106 CFU/ml of yeast spread on Mueller-Hinton Agar (MHA) and Saboraud Dextrose Agar (SDA) respectively. The disc (6mm in diameter) were impregnated with test fractions at concentrations 100µg/disc. The methanol extract and the other fractions did not show any anticandidal activity.

Tested organism

Zone of inhibition (mm)

Water

MeOH

EtOAc

CHCl3

n-hexane

Escherichia coli

7

18

15

8

18

Staphylococcus aureus

5

10

10

5

15

Positive control: Ampicillin (10µg) >19–10mm sensitive (It was measured all bacterial strains)

References: [1] Baytop, T. (1999) „Therapy with Medicinal Plants in Turkey (Past and Present)“, 2nd ed. pp. 325–326, Nobel Tıp Kitabevleri, İstanbul. [2] Ietswaart, J.H. (1982) Origanum L., „Flora of Turkey and the East Aegean Islands“, Vol. 7, pp. 297–313, University Press, Edinburgh (edited by P.H. Davis) [3] Özgen, U., et. al. (2005) Phytochemical Studies on Origanum acutidens (Hand.-Mazz.) Iestwaart, 53rd Annual Congress of GA, Florence, Italy, August 21st-25th.