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DOI: 10.1055/s-2007-986970
Antimicrobial studies on Origanum acutidens (Hand.-Mazz.) Ietswaart
Many Origanum species (Lamiaceae) known as „Kekik, kekik otu, keklik otu, mercanköşk“ are used by the public especially as a spice and for its several medicinal effects in Turkey [1]. O. acutidens is an endemic species and grows mainly in Central and East Anatolia [2]. Ursolic and oleanolic acid; rosmarinic and lithospermic acid; vicenin-2, betulalbuside A, 8-OH-linaloyl glucoside were isolated from aerial parts of O. acutidens [3]. In this study, the antibacterial and anticandidal activity of O. acutidens methanol extract and fractions (n-hexane, CHCl3, EtOAc, aqueous) prepared from methanol extract were evaluated against clinical bacterial and candida strains. Antimicrobial test was then carried out by disc-diffusion method using suspension containing 108 CFU/ml of bacteria, 106 CFU/ml of yeast spread on Mueller-Hinton Agar (MHA) and Saboraud Dextrose Agar (SDA) respectively. The disc (6mm in diameter) were impregnated with test fractions at concentrations 100µg/disc. The methanol extract and the other fractions did not show any anticandidal activity.
Tested organism |
Zone of inhibition (mm) |
||||
Water |
MeOH |
EtOAc |
CHCl3 |
n-hexane |
|
Escherichia coli |
7 |
18 |
15 |
8 |
18 |
Staphylococcus aureus |
5 |
10 |
10 |
5 |
15 |
Positive control: Ampicillin (10µg) >19–10mm sensitive (It was measured all bacterial strains)
References: [1] Baytop, T. (1999) „Therapy with Medicinal Plants in Turkey (Past and Present)“, 2nd ed. pp. 325–326, Nobel Tıp Kitabevleri, İstanbul. [2] Ietswaart, J.H. (1982) Origanum L., „Flora of Turkey and the East Aegean Islands“, Vol. 7, pp. 297–313, University Press, Edinburgh (edited by P.H. Davis) [3] Özgen, U., et. al. (2005) Phytochemical Studies on Origanum acutidens (Hand.-Mazz.) Iestwaart, 53rd Annual Congress of GA, Florence, Italy, August 21st-25th.