Intestinal Prostaglandin E₂ Expression in Rats with Obstructive Jaundice

Abstract

Aim: How obstructive jaundice causes the intestinal barrier to be injured is still controversially discussed. In this study, we hypothesize that intestinal prostaglandin E₂, a cytoprotective factor, may be affected by the bile duct obstruction. Materials and Methods: Four groups of Wistar-Albino rats were used: in Groups 1 and 3, the rats underwent a sham operation. In Groups 2 and 4, the common bile duct was doubly ligated. Relaparotomy was performed after one week in Groups 1 and 2, and after two weeks in Groups 3 and 4, and specimens of the jejunum, ileum and liver were obtained for intestinal PGE₂ analysis and histopathological evaluation. Results: Jejunal and ileal PGE₂ levels had significantly decreased in two-week bile duct-ligated rats compared to one-week ligated rats and the sham group (p < 0.01). Tissue injury scores (Chiu score) of the ileum were significantly higher in the two-week and one-week ligated rats than in the controls (p < 0.01 and p < 0.05, respectively). The jejunal injury score was significantly higher in the two-week ligated rats compared to controls (p < 0.05). The ileal and jejunal injury scores were higher in the two-week ligated rats than in the one-week ligated rats (p < 0.01 and p < 0.05, respectively). Precirrhotic fibrosis was detected in all two-week ligated rats, but in only 7 of 10 one-week ligated rats. Conclusions: Obstructive jaundice associated with intestinal tissue injury and precirrhotic changes leads to reduced intestinal PGE₂-levels, suggesting an adverse effect on the intestinal cytoprotective process.

Key words

obstructive jaundice - intestine - prostaglandin

References

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