Planta Med 2008; 74(7): 730-735
DOI: 10.1055/s-2008-1074521
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Antioxidant Properties of Grape Seed Extract on Human Lymphocyte Oxidative Defence

Miroslava Stanković1 , Vele Tešević2 , Vlatka Vajs3 , Nina Todorović3 , Slobodan Milosavljević2 , Dejan Gođevac3
  • 1Institute of Nuclear Science ”Vinča”, University of Belgrade, Belgrade, Serbia
  • 2Faculty of Chemistry, University of Belgrade, Belgrade, Serbia
  • 3Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia
Further Information

Publication History

Received: November 30, 2007 Revised: March 7, 2008

Accepted: March 13, 2008

Publication Date:
21 May 2008 (online)


The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and 13C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1 - 6 μg/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 μg/mL induced a significant decrease in the frequency of micronuclei by 40 %, reduction of malonyldialdehyde production by 30 %, while a concentration of 5 μg/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.


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