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DOI: 10.1055/s-2008-1074529
© Georg Thieme Verlag KG Stuttgart · New York
Cytotoxic Activity of Diterpenes and Extracts of Juniperus brevifolia
Publication History
Received: December 14, 2007
Revised: March 24, 2008
Accepted: March 31, 2008
Publication Date:
16 May 2008 (online)
Abstract
The hexane, dichloromethane, acetone and ethanol extracts of the leaves, bark and wood of Juniperus brevifolia were subjected to screening against human tumour cells lines from the cervix (HeLa) and larynx (Hep-2) in two different stages of the cell cycle. The dichloromethane and the chloroform-soluble portions of the acetone extracts of the leaves were active against both tumour cell lines. Chemical investigation of one of the most active extracts (dichloromethane extract from leaves) by preparative chromatography afforded compounds 1 - 10. Among these, compounds 1 - 3 were isolated for the first time from the Juniperus genus (18-hydroxyferruginol, 6,7-dehydrototarol and 7α-hydroxytotarol) while 4 and 5 are rare as natural products corresponding to (E)- and (Z)-isomers of 8α-hydroxy-labda-13(16),14-dien-19-yl-coumarate, respectively. The structures of all compounds were established based on various spectral data. The cytotoxic activity was evaluated for the first time for compounds 1 - 5 against HeLa and Vero cell lines in lag and log phases of growth. Compound 5 was shown to be active and selective to HeLa in the log phase.
Abbreviations
AB: acetone extract from bark
ALIC: chloroform-insoluble portion of the acetone extractfrom leaves
ALSC: chloroform-soluble portion of the acetone extract from leaves
AW: acetone extract from wood
DL: dichloromethane extract from leaves
DMSO: dimethyl sulfoxide
DW: dichloromethane extract from wood
EB: ethanol extract from bark
EL: ethanol extract from leaves
EW: ethanol extract from wood
HB: hexane extract from bark
HeLa: human carcinoma of the cervix
Hep-2: human carcinoma of the larynx
HL: hexane extract from leaves
Vero: African green monkey kidney cell line
Key words
Juniperus brevifolia - Cupressaceae - HeLa - Hep-2 - diterpenes
- Supporting Information for this article is available online at
- Supporting Information .
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