Steroid Metabolism in Stationary Phase Cell Suspensions of Dioscorea deltoidea

M. R. Heble; E. J. Staba

doi:10.1055/s-2008-1075016

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Planta Med 1980; 40: 124-128
DOI: 10.1055/s-2008-1075016

Research Articles

Steroid Metabolism in Stationary Phase Cell Suspensions of Dioscorea deltoidea

M. R. Heble¹ , E. J. Staba

College of Pharmacy, University of Minnesota, Minneapolis, Minnesota, U.S.A.
¹Bio-organic Division, Bhabha Atomic Research Centre, Bombay, India

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Publication History

Publication Date:
29 April 2008 (online)

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Abstract

Cell suspensions of Dioscorea deltoidea were studied in the stationary phase of growth in Murashige and Skoog's revised tobacco medium depleted of either carbohydrates, growth regulators, nitrogen or supplemented with hydroxyurea. Stationary phase cells and media were collected after ten days incubation and analyzed by TLC and GLC. The concentration of phytosterols and diosgenin was significantly greater in stationary phase cells incubated in media depleted of carbohydrates.

Key Word Index

Dioscorea deltoidea - Dioscoreaceae - Suspension Culture - Stationary Phase Cells - Steroids.

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