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Planta Med 2020; 86(08): 556-564
DOI: 10.1055/a-1147-9196
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Network Pharmacological Screening of the Active Ingredients and Hypoglycemic Effect of Isodon rubescens in the Treatment of Diabetes

Xue Jintao
1   School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan Province, PR China
,
Yu Shasha
1   School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan Province, PR China
,
Wang Jincai
3   Department of Pharmacy, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan Province, PR China
,
Li Chunyan
1   School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan Province, PR China
2   Sanquan College of Xinxiang Medical University, Xinxiang, Henan Province, PR China
,
Yang Mengya
1   School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan Province, PR China
,
Shi Yongli
1   School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan Province, PR China
› Author AffiliationsSupported by: National Natural Science Foundation of China for Youth Program 81703458
Supported by: the National Natural Science Foundation of China U1804175
Further Information

Publication History

received 01 January 2020
revised 12 March 2020

accepted 25 March 2020

Publication Date:
15 April 2020 (online)

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Abstract

This study was firstly to study the relationship of “ingredient-target-pathway” and the pharmacological effects of Isodon rubescens for the treatment of diabetes. Based on a network pharmacology method, 138 active ingredients of Isodon rubescens were screened from the relative literatures, and their targets were confirmed by comparing these with the hypoglycemic targets in the DrugBank database. Results showed that Isodon rubescens contained 25 hypoglycemic ingredients, such as rabdoternin A, rabdoternin B, and epinodosinol. These ingredients could activate 6 hypoglycemic targets, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), integrin α-L (ITGAL), integrin β-2 (ITGB2), progesterone receptor (PGR), glucocorticoid receptor (NR3C1), and nuclear receptor subfamily 1 group I member 2 (NR1I2). These targets were involved in 94 signaling pathways, such as the Rap1, PI3K-Akt, and HIF-1 signaling pathways. The cell viability showed that the human umbilical vein endothelial cells (HUVECs) treated with alcohol extract (1.00 g/L) and the water extract (0.13 – 0.50 g/L) exhibited high viability compared to the model group (p < 0.05), respectively. 0In animal experiments, the rats treated with water extract of Isodon rubescens showed significant hypoglycemic effects compared to rats in the model group (p < 0.05). Overall, this approach provides an efficient strategy to explore hypoglycemic ingredients of Isodon rubescens and other traditional Chinese medicine.

Key words

diabetes - network pharmacology - Isodon rubescens - Lamiaceae - active ingredient - target - signaling pathway

Supporting Information

    Supplemental Table 1S. Chemical structural information of 138 ingredients in Isodon rubescens is included in supplemental information.

  • Supporting Information
 

  • References

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