Abstract
Wound infections are a significant medical problem, with Staphylococcus species being one of the most common etiological factors. Treatment is complex due
to bacterial antibiotic resistance and the ability to form a biofilm. The aim of this
study was to determine the drug susceptibility of the clinical isolates of Staphylococcus species obtained from wound infections, assess their ability to form a biofilm in vitro, evaluate the level of extracellular slime synthesis, and test the antistaphylococcal
properties of selected plant extracts against plate-cultured bacteria and activity
against mature biofilms. A total of 20 Staphylococcus strains were evaluated. The antibiotic susceptibility and sensitivity of the strains
to the tested extracts were determined using the disc diffusion method. The production
of extracellular bacterial slime was assessed using Congo Red agar plates. The biofilm
formation and the effect of plant extracts on the biofilm
layer were examined using the MTT method on polystyrene microtiter plates. Diverse
drug susceptibility profiles, slime production ability, and in vitro biofilm formation were observed among the tested strains. It was found that aqueous
extracts from the Serpylli herba, Arctii lappae folium, Taraxaci folium, and Galii
aparini herba showed antibacterial activity against some of the tested strains. In
contrast, their antibiofilm activity was not confirmed. Among all tested preparations,
the most promising antimicrobial extracts in both planktonic cultures and biofilm
were Thymus serpyllum herb and Taraxacum officinale leaf extracts. These results allowed us to conclude their potential application in
medicine to support the treatment of challenging wound conditions.
Keywords
Asteraceae -
Galium aparine
- Lamiaceae - Rubiaceae -
Taraxacum officinale
-
Thymus serpyllum
