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Planta Med
DOI: 10.1055/a-2573-7718
Original Papers

Chemical Structural Elucidation and Immunomodulatory Activity of a New Polysaccharide from Saposhnikoviae Radix

Yifang Cui‡
1   School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
,
Haitao Fan‡
2   College of Bioengineering, Beijing Polytechnic, Beijing, China
,
Meng Sun
1   School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
,
Xinyang He
1   School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
,
Jie Li
1   School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
,
Guangzhong Tu
3   Beijing Institute of Microchemistry, Beijing, China
,
Yanyan Jiang 
1   School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
4   The Key Research Laboratory of “Exploring Effective Substance in Classic and Famous Prescriptions of Traditional Chinese Medicine”, The State Administration of Traditional Chinese Medicine of the Peopleʼs Republic of China, Beijing, China
,
Bin Liu
1   School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China
4   The Key Research Laboratory of “Exploring Effective Substance in Classic and Famous Prescriptions of Traditional Chinese Medicine”, The State Administration of Traditional Chinese Medicine of the Peopleʼs Republic of China, Beijing, China
› Author AffiliationsThis research was financially supported by the National Natural Science Foundation of China (No. 82074283).
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Abstract

The chemical structure and immunomodulatory activity of a new homogeneous polysaccharide, SP4002501, isolated from Saposhnikoviae Radix (SR), were investigated. Purification of SP4002501 was performed by DEAE-Cellulose and Sepharose CL-6B column chromatography. The monosaccharidic constituents were identified as rhamnose (Rha), galacturonic acid (GalA), galactose (Gal), and arabinose (Ara) with a molar ratio of 3.7: 86.6: 2.7: 7.1. According to the methylation analysis, partial hydrolysis, FT-IR, and NMR analysis, SP4002501 had a backbone of polygalacturonic acid units with a small amount of galactose (Gal). Side chains are connected to C-3 of galactose (Gal) and consist of rhamnose (Rha), galacturonic acid (GalA), galactose (Gal), and arabinose (Ara), with arabinose (Ara) as terminal sugar. Biological activity assessment suggests that SP4002501 exhibits immunomodulatory activity through promoting macrophage proliferation and phagocytosis.

Keywords

polysaccharide - Saposhnikoviae Radix - Saposhnikovia divaricata - Apiaceae - chemical structure - immunomodulatory activity

Supporting Information

    Homogeneity test results, monosaccharide composition chromatogram, infrared spectrum, TIC of methylated hydrolysates, chromatogram of partial hydrolyzed product, ESI-MS2 spectrometry of partial enzymatic hydrolysis production, and possible cleavage pathway are available as Supporting Information.

  • Supporting Information


Publication History

Received: 12 September 2024

Accepted after revision: 02 April 2025

Accepted Manuscript online:
03 April 2025

Article published online:
08 May 2025

© 2025. Thieme. All rights reserved.

Georg Thieme Verlag KG
Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany

 

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