Metabolism of Hibifolin by Human Intestinal Bacteria

Abstract

Hibifolin, the highest-content bioactive flavonoid of the flowers of Abelmoschus manihot, was incubated with human intestinal bacteria, and four metabolites (1–4) were obtained from the incubated solution by chromatographic methods. The structures of the four metabolites were elucidated as gossypetin 8-O-β-D-4′′-deoxy-Δ ^4′′-glucuropyranoside (1), gossypetin (2), quercetin (3), and 8-methoxy-quercetin (4), respectively, on the basis of UV, NMR, and MS data. Metabolite 1 was obtained as a new compound with a specific β-D-4′′-deoxy-Δ ^4′′-glucuropyranosyl moiety, which was formed through a unique and novel metabolic pathway that has not been reported previously.

Key words

hibifolin - human intestinal bacteria - metabolites - gossypetin 8‐O‐β‐D‐4′′‐deoxy‐Δ ^4′′‐glucuropyranoside - Abelmoschus manihot - Malvaceae

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