Abstract
A validated HPLC method is described for determination of glaucocalyxin A in rat plasma
using liquid-liquid extraction and UV absorbance detection. The extraction recovery
of glaucocalyxin A ranged from 81.72 % to 79.25 %, the linear range was 0.2–10 µg/mL,
and r was 0.9984. A pharmacokinetic analysis of glaucocalyxin A after intravenous administration
in rats revealed that glaucocalyxin A followed a two-compartment open model. The values
of t1/2α and t1/2β were 4.327 min and 28.56 min, respectively, and the area under the plasma concentration-time
curve (AUC) was 222.744 µg · min · mL−1. The method developed was simple, rapid and specific, and an accurate way of investigating
the pharmacokinetics of a diterpenoid in plasma.
Key words
pharmacokinetics - glaucocalyxin A - HPLC
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Dr. Luye Cao
Department of Traditional Chinese Medicine
Food and Drug Vocational College of Guangdong
Longdong Northern Street, tianhe area
Guangzhou 510520
People's Republic of China
Phone: + 86 20 28 85 49 35
Fax: + 86 20 28 85 49 10
Email: yncly001@yahoo.com.cn
