Pharmacokinetic Analysis of Glaucocalyxin A in Rat Plasma by Liquid-Liquid Extraction and HPLC

Abstract

A validated HPLC method is described for determination of glaucocalyxin A in rat plasma using liquid-liquid extraction and UV absorbance detection. The extraction recovery of glaucocalyxin A ranged from 81.72 % to 79.25 %, the linear range was 0.2–10 µg/mL, and r was 0.9984. A pharmacokinetic analysis of glaucocalyxin A after intravenous administration in rats revealed that glaucocalyxin A followed a two-compartment open model. The values of t_1/2α and t_1/2β were 4.327 min and 28.56 min, respectively, and the area under the plasma concentration-time curve (AUC) was 222.744 µg · min · mL⁻¹. The method developed was simple, rapid and specific, and an accurate way of investigating the pharmacokinetics of a diterpenoid in plasma.

Key words

pharmacokinetics - glaucocalyxin A - HPLC

References

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