Planta Medica, Table of Contents Planta Med 2010; 76(10): 1007-1010DOI: 10.1055/s-0029-1240911 Natural Product ChemistryLetters© Georg Thieme Verlag KG Stuttgart · New YorkChemical Constituents of Alnus firma and Their Inhibitory Activity on Lipopolysaccharide-Induced Nitric Oxide Production in BV2 MicrogliaMin A. Lee1 , Hee Kyoung Lee2 , Seung Hyun Kim2 , Young Choong Kim1 , Sang Hyun Sung1 1College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea 2Institute for Life Science, ElcomScience Co., Ltd., Seoul, Korea Recommend Article Abstract Buy Article Corrected by:Erratum for: Chemical Constituents of Alnus firma and Their Inhibitory Activity on Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglia eFirstDOI: 10.1055/s-0030-1249808 Abstract A methanolic extract of Alnus firma barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6′-O-syringoyl-β-D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6′-O-vanilloyl-β-D-glucopyranoside (2), were isolated from the barks of A. firma using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (5–15). Among these compounds, 1–4 and 6–11 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10 µM to 100 µM. Key words Alnus firma (Betulaceae) - phenolic glycosides - diarylheptanoids - nitric oxide - BV2 microglia - lipopolysaccharide Full Text References References 1 Bredt D S, Snyder S H. Nitric oxide: a physiologic messenger molecule. Annu Rev Biochem. 1994; 63 175-195 2 Bubgon O, Schaad N C, Schorderet M. Nitric oxide modulates endogenous dopamine release in bovine retina. 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Chemical constituents isolated from Polygala japonica leaves and their inhibitory effect on nitric oxide production in vitro. J Enzyme Inhib Med Chem. 2009; 24 230-233 Prof. Dr. Sang Hyun Sung College of Pharmacy and Research Institute of Pharmaceutical SciencesSeoul National University 599 Gwanak-ro Gwanak-gu Seoul 151–742 Korea Phone: + 82 28 80 78 59 Fax: + 82 28 77 78 59 Email: shsung@snu.ac.kr Supplementary Material Supplementary Material www.thieme-connect.de/ejournals/toc/plantamedica
