Keywords
gastroschisis - enteric nervous system - interstitial cells of Cajal - experimental
models
Introduction
Gastroschisis is a congenital abdominal wall defect that poses several challenges
to surgeons and pediatricians. In this condition, the small bowel is herniated through
the abdominal wall defect and is directly exposed to amniotic fluid in utero.[1]
[2] After birth, surgery and supportive care must be provided promptly. Following surgical
repair, many patients exhibit severe intestinal dysmotility that requires prolonged
parenteral nutrition.[1]
[2]
[3]
[4] These neonates are subsequently at risk for sepsis and parenteral nutrition-induced
liver disease. Although the mortality rate of live-born gastroschisis patients has
decreased over time and is now less than 10%, the morbidity rate remains high as a
result of prolonged intestinal dysmotility, associated intestinal atresia, short bowel
syndrome, and iatrogenic adverse events.[1]
[2]
[3]
[4] The initiation and regulation of small-bowel motility require the normal function
of several cell populations. The enteric nervous system is a neuronal network located
all along the gut that regulates intestinal peristalsis and secretion.[5] Within the small-bowel wall, the enteric nervous system has two components, the
submucosal plexus and the myenteric plexus, both made up of neurons and glial cells
derived from the neural crest.[5] The interstitial cells of Cajal (ICC) are cells derived from the mesoderm and serve
as electrical pacemakers in the gut wall, generating and propagating the electrical
signal to the smooth muscle.[6] The smooth muscle cells are the final effector cells responsible for small-bowel
motility.
Given that severe intestinal dysmotility is a prominent feature in neonates with gastroschisis,
we investigated the hypothesis that gastroschisis may be associated with impairment
of the enteric nervous system or ICC network. To this end, we used a previously established
rat model of gastroschisis[7] and assessed the phenotype of the enteric nervous system and ICC network in the
eviscerated small bowel.
Methods
Rat Model of Gastroschisis
Animal care and use complied with French regulations regarding experiments on animals.
Time-dated pregnant rats were purchased and two were housed to a cage on a 12-hour
dark cycle/12-hour light cycle with food and water ad libitum in the laboratory animal
facility of the INSERM Research Center (Saint-Antoine Hospital, Paris, France).
Gastroschisis was created surgically in rat fetuses as previously described by Correia-Pinto
et al.[7] Briefly, pregnant rats were anesthetized on gestational day 18 using intramuscular
ketamine (Panpharma, France) and chlorpromazine (Sanofi-Aventis, France), with additional
inhalation of sevoflurane (Abbott, UK) if needed. The uterus was exposed through a
midline laparotomy and opened in the middle of a 7/0 polydioxanone (Ethicon, Belgium)
purse string. Then, under surgical microscope visualization, an incision was performed
in the right or left lower abdominal quadrant and gentle pressure was applied to the
fetal abdomen to extrude bowel loops through the incision. The other fetuses received
either only manipulation (sham-operated group) or no intervention (control group).
Warm saline solution was infused into the amniotic cavity and the purse string was
closed. The laparotomy was closed in two layers with continuous absorbable sutures.
To compensate for the high mortality rate expected after gastroschisis creation, we
performed the gastroschisis and sham procedures in 80 and 30 fetuses, respectively.
For each dam, we created a diagram of the uterus showing the position and group (gastroschisis,
sham, or control) of each fetus.
Harvesting and Macroscopic Assessment of Small Bowel
The entire small bowel of each rat fetus in each of the three groups (gastroschisis,
sham, and control) was harvested on day 22 of gestation under general anesthesia.
Fetuses that died in utero or exhibited necrosis of the eviscerated small bowel were
excluded from the analysis. Eviscerated and noneviscerated small bowel specimens were
assessed and processed separately. Presence of a fibrous coating or peel on the eviscerated
small bowel in the fetuses with gastroschisis was assessed macroscopically. The length
of the small bowel was measured in millimeters and the weight in mg; these values
were used to compute the ratio of intestinal weight over intestinal length (IW/L).
In some instances extensive peel formation with severe edema and retraction precluded
measurement of small-bowel length. Therefore, we defined a weight score combining
intestinal weight/intestinal length (IW/L) values and macroscopic assessment of peel
formation. IW/L was converted to a weight score as follows: 1, IW/L = 0 to 15 mg/cm;
2, IW/L = 15 to 30 mg/cm; 3, IW/L = 30 to 45 mg/cm; and 4, IW/L > 45 mg/cm or major
peel preventing length measurement. Thus the highest possible score (4) was either
IW/L > 45 mg/cm or major peel preventing length measurement, which allowed the evaluation
of all small bowels including those with severe damage. After macroscopic assessment,
a small-bowel specimen from each fetus was collected, fixed in 10% formalin, and embedded
in paraffin.
Histology and Immunohistochemistry
Serial 5-μm transversal sections, at least 50 per specimen, were obtained from the
paraffin-embedded small-bowel specimens and processed for hematoxylin-eosin staining
or immunohistochemistry. Each slide had an identification number with no reference
to the group of the fetus, to keep assessor “blind” during analysis. Immunochemistry
was performed according to the standard procedures. Briefly, paraffin sections were
air-dried overnight in a 50°C oven, deparaffinized, and rehydrated through decreasing
alcohol series down to distilled water. The sections were incubated for 30 minutes
with peroxidase-blocking reagent (DakoCytomation, Glostrup, Denmark). Heat-induced
epitope retrieval was performed by immersion in antigen unmasking solution (Vector
Laboratories, Burlingame, California, United States) and microwave heating for all
stains except α-smooth-muscle actin. The sections were incubated with the primary
antibody for 12 hours at 4°C then with the secondary poly-HRB conjugate antibody (Zymed
Invitrogen, Carlsbad, California, United States) for 30 minutes at room temperature.
The sections were rinsed in phosphate-buffered saline, and immunoreactivity was detected
at room temperature by addition of DAB substrate (ref SK-4100, Vector Laboratories)
for 4 minutes. Counterstaining was done with Harris hematoxylin.
The following markers were studied by immunohistochemistry ([Table 1]): α-smooth-muscle actin (smooth muscle cells), GFAP (intracytoplasmic filamentous
protein found in the cytoskeleton of astrocytes and enteric glial cells), S100 protein
(a calcium-binding protein abundant in glial cells of the central and peripheral nervous
system), Hoxb5 (enteric neuroblasts and neuronal precursors), and c-kit (ICCs). We
performed a preliminary titration and cross-reactivity study of the antibodies on
small-bowel specimens from control rats before using them in the study.
Table 1
Antibodies used for the immunohistochemistry studies of fetal rat small-bowel specimens
Labeled cell type
|
Antibody
|
Supplier and references
|
Dilution
|
Smooth muscle cells
|
Mouse antihuman α-smooth-muscle actin[a]
|
Dako[b], monoclonal purified, clone 1A4, ref. M0851
|
1:100
|
Enteric neurons
|
Rabbit antirat Hoxb5
|
Polyclonal, generated by MV Volpe, Tufts Medical Center, Boston, Massachusetts, United
States
|
1:100
|
Glial cells
|
Rabbit anticow S100B[a]
|
Dako[b], polyclonal purified, ref. Z0311
|
1:400
|
Glial cells
|
Rabbit anticow GFAP[a]
|
Dako[b], polyclonal purified, ref. Z0334
|
1:10
|
Interstitial cells of Cajal
|
Rabbit antihuman C-KIT[a]
|
Dako[b], polyclonal purified, ref. A4502
|
1:50
|
a Cross-react with same rat antigen.
b DakoCytomation, Glostrup, Denmark.
Light Microscopy: To assess small-bowel inflammation, in addition to peel formation, the IW/L ratio,
and the weight score, we used five continuous variables: total thickness of the small-bowel
wall (μm), thickness of the small-bowel muscular layer (μm), thickness of the small-bowel
submucosa (μm), inner width of the small-bowel villi (chorion of the villus measured
between the epithelium basement membranes on each side, in μm), and outer width of
the small-bowel villi (total width of the villus measured between the epithelium apical
membranes on each side, in μm).
These variables were evaluated by observing serial 5-μm transversal sections under
the light microscope, and analysis was performed on circular sections of small bowel,
at least 10 per specimen. The investigator assessing the sections was “blind” to the
group of the specimen. Digital images were obtained on a Leica DMR microscope (Wetzlar,
Germany) equipped with a charge-coupled device camera (3CCD Color Video Camera Model
DXC-950P, Sony, Japan) and recorded with Tribvn version 1.3 software (Châtillon, France).
The overall structure of the small-bowel layers was assessed and morphometric variables
were obtained using image-analysis software (SigmaScan Pro 5 Aspire Software International,
Ashburn, Virginia, United States). For each variable in each fetus, the mean of 10
measurements was used for the study.
The enteric nervous system was assessed by a qualitative analysis of the structure
of the myenteric plexus lying between the two muscular layers of the small bowel after
α-smooth-muscle actin immunostaining and hematoxylin counterstaining. Stage of development
of the myenteric plexus was graded from 1 (mature) to 3 (very immature) comparatively
with specimens from E16 to E22 control fetuses ([Fig. 1]). Grade 1 (mature) myenteric plexus was observed in E21-E22 control fetuses and
defined as a discontinuous belt of well-defined ganglia with round cells, grade 2
(immature) was observed in E19-E20 control fetuses and defined as a discontinuous
belt with ovoid cells, and grade 3 (very immature) was observed in E16-E18 control
fetuses and defined as a continuous belt with ovoid or spindle-shaped cells. Expression
of Hoxb5, GFAP, and S100 proteins was assessed after immunostaining and graded using
a semiquantitative 3-point scale (1, weak expression; 2, medium expression; and 3,
strong expression). For each marker in each fetus, at least 10 transverse sections
were evaluated. Strong expression was defined as the presence of highly positive cells
in all myenteric plexus. Medium expression was defined as the presence of highly positive
cells in at least 50% of myenteric plexus per circular section. Weak expression was
defined as the presence of positive cells in at least one myenteric plexus per circular
section.
Figure 1 Small bowel from control fetuses harvested from E16 to E21, magnification x10 (panel
A–C) or x20 (panel D–F). Immunostaining with anti-α-smooth-muscle actin antibody.
ICCs were evaluated using light microscopy after c-kit immunostaining and hematoxylin
counterstaining. The presence and appearance of the ICC network were recorded. The
ICC network was considered normal when the cells showed both strong c-kit expression
and a typical spindle shape with thin branches extending around the myenteric plexus.
The ICC network was considered abnormal when either criterion was absent.
Statistical Analysis
The study variables were as follows: peel formation (yes/no), mean IW/L, mean weight
score, mean total thickness of the small bowel wall, mean thickness of the muscular
layers of the small bowel wall, mean thickness of the submucosa of the small bowel
wall, overall structure of the small bowel, mean inner thickness of the small bowel
villi, mean outer thickness of the small bowel villi, maturity of the myenteric plexus,
Hoxb5 expression (yes/no), GFAP expression (yes/no), S100 protein expression (yes/no),
Hoxb5 expression level (3-point scale), GFAP expression level (3-point scale), and
S100 protein expression level (3-point scale).
All statistical analyses were done using SPSSversion 15.0 for Windows (2006, SPSS
Inc., Chicago, Illinois, United States). Peel formation between groups was compared
using the nonparametric Kruskal–Wallis and Mann–Whitney tests. For comparisons of
numerical data, we used one-way ANOVA with the Bonferroni post hoc test. p values of less than 0.05 were considered significant.
Results
Survival rates after gastroschisis creation or sham procedure were 14% (11 fetuses
/80) and 73% (22 fetuses /30), respectively. The study population included 11 gastroschisis
fetuses, 22 sham fetuses, and 9 control fetuses.
Peel Formation
Peel formation was observed in 4 of the 11 gastroschisis fetuses (36%). The macroscopic
appearance of the small bowel was normal in all sham and control fetuses ([Table 2]).
Table 2
Peel assessment, morphometric analysis, and myenteric plexus maturation in the fetuses
with gastroschisis, sham fetuses, and control fetuses
|
Controls
|
Shams
|
Gastroschisis
|
N
|
9
|
22
|
11
|
Peel formation
|
0
|
0
|
4 (36%)
|
Weight to length ratio (mg/cm)
|
Mean (SD)
|
9.18 (2.70)
|
12.61 (3.60)
|
21.25 (12.71)[a]
|
Weight score (N)
|
Weight score = 1
|
9
|
18
|
3
|
Weight score = 2
|
0
|
4
|
4
|
Weight score = 3
|
0
|
0
|
1
|
Weight score = 4
|
0
|
0
|
3 (27%)
|
Weight score: mean (SD)
|
1 (0)
|
1.18 (0.39)
|
2.55 (1.21)[b]
|
Small bowel thickness (µm)
|
Total: mean (SD)
|
57.44 (4.11)
|
58.26 (6.11)
|
78.44 (18.64)[c]
|
Muscular layers: mean (SD)
|
22.90 (2.74)
|
23.06 (4.00)
|
30.23 (8.04)[d]
|
Submucosa: mean (SD)
|
14.45 (2.50)
|
13.84 (2.63)
|
19.98 (7.82)[a]
|
Villi (µm)
|
Inner width: mean (SD)
|
12.44 (1.05)
|
11.14 (1.43)
|
12.24 (3.01)
|
Outer width:mean (SD)
|
62.81 (7.28)
|
62.02 (9.32)
|
54.96 (9.46)
|
Myenteric plexus
|
Mature: N (%)
|
7 (78%)
|
20 (91%)
|
0 (0%)
|
Immature: N (%)
|
1 (11%)
|
2 (9%)
|
6 (55%)
|
Very immature: N (%)
|
1 (11%)
|
0 (0%)
|
5 (45%)
|
a
p < 0.05.
b
p < 0.001.
c
p < 0.0001.
d
p < 0.01 for comparison with control and sham fetuses, respectively.
In 7 of the 11 fetuses with gastroschisis, the entire small bowel was outside the
abdominal cavity, which contained the stomach and colon. Therefore, we were unable
to perform a paired analysis of eviscerated and intra-abdominal small bowel. In the
four fetuses with incomplete small bowel evisceration, the intra-abdominal small bowel
showed no peel formation.
Intestinal Weight/Intestinal Length Ratio, and Weight Score
The mean IW/L ratio was significantly higher (p < 0.05) in fetuses with gastroschisis (21.25 mg/cm) than in sham fetuses (12.61 mg/cm)
and control fetuses (9.18 mg/cm). In one gastroschisis fetus, extensive peel formation
with severe edema and retraction precluded measurement of small-bowel length. Therefore,
we defined a weight score combining IW/L values and macroscopic assessment of peel
formation which allowed the evaluation of all small bowels. The mean weight score
and the proportion of fetuses whose weight score was abnormal (i.e., greater than
1) were higher among the gastroschisis fetuses (2.55 and 73%, respectively) compared
with the sham fetuses (1.18 and 18%, respectively; p < 0.001) and control fetuses (1.00 and 0%, respectively; p < 0.001). ([Table 2])
Small Bowel Wall Thickness and Histology
Although the overall gross anatomy of the small bowel was normal in the fetuses with
gastroschisis, the subjective assessment consistently suggested thickening of the
bowel wall compared with sham and control fetuses. This assessment was confirmed by
the measurements of the thickness of the small bowel wall and showed that the mean
total thickness of the small-bowel wall was 78.44 µm in fetuses with gastroschisis
compared with 58.26 µm in sham fetuses and 57.44 µm in control fetuses (p < 0.0001 for both comparisons). We also asked whether increased small bowel thickness
in fetuses with gastroschisis was restricted to specific layers of the small bowel
wall. To answer this question, each layer of the small bowel wall was measured for
each sample. Mean thickness of both the muscular layer and the submucosa of the small-bowel
wall were higher in the fetuses with gastroschisis than in the sham fetuses and control
fetuses.
The overall structure and thickness of the small-bowel villi was not different between
gastroschisis, sham, and control fetuses. ([Table 2] and [Fig. 2A–C])
Figure 2 Panel A–C: Mean thickness of the small-bowel wall (micrometers). The error bars indicate
the standard deviations. The mean total (A), muscular (B), and submucosa (C) thickness
of the small bowel wall were significantly higher in the fetuses with gastroschisis
compared with the sham fetuses and control fetuses (p < 0.0001, p < 0.01, and p < 0.05, respectively). Panel D: Morphology of the myenteric plexus, categorized as
mature, immature, or very immature (see Methods section for definitions). Panel E:
Interstitial cells of Cajal: intensity of c-kit immunostaining in the three groups.
Myenteric Plexus
The myenteric plexus were visible in the small-bowel specimens from all fetuses with
gastroschisis. However, none of these fetuses exhibited normal myenteric plexus. Thus,
of the gastroschisis fetuses, 55% had an aspect similar to the immature myenteric
plexus of E19-E20 fetuses and 45% to the very immature myenteric plexus observed in
E16-E18 fetuses, compared with 9 and 0% in the sham fetuses, respectively, and 11
and 11% in the control fetuses, respectively ([Table 2] and [Figs. 2D] and [3]).
Figure 3 Small bowel on E22, magnification x20. Immunostaining with anti-α-smooth-muscle actin
antibody. Arrows indicate myenteric plexus ganglia. Panel A, B: Small bowel from control
fetuses with mature myenteric plexuses. Panel C, D: Small bowel from gastroschisis
fetuses with immature myenteric plexuses. Panel E, F: Small bowel from gastroschisis
fetuses with very immature myenteric plexuses.
Hoxb5, a marker for enteric neuroblasts and neuronal precursors, was expressed in
myenteric plexuses in all groups ([Fig. 4A–C]). In the gastroschisis group, 70% of fetuses displayed Hoxb5 expression in the myenteric
plexus. This proportion was not different from the proportions observed in sham and
control groups.
Figure 4 Panel A, B: Neuroblast precursors in small bowel in a sham fetus and a gastroschisis
fetus: Hoxb5 immunostaining, magnification x40. Panel C: Neuroblast precursors: mean
intensity of Hoxb5 immunostaining in the three groups. Panel D, E: Enteric glial cells
in a sham fetus and a gastroschisis fetus: protein S100 immunostaining, magnification
x40. Panel F: Enteric glial cells: mean intensity of protein S100 immunostaining in
the three groups. Panel G, H: Enteric glial cells: GFAP immunostaining, magnification
x40. Panel I: Enteric glial cells: mean intensity of GFAP immunostaining in the three
groups. Hoxb5 and S100 (panel C and F) expression levels in the myenteric plexus of
gastroschisis fetuses were not different from the expression levels observed in sham
and control groups. The mean GFAP expression level (panel I) was higher in the gastroschisis
group than in the sham and control groups (p < 0.002). The error bars indicate the standard deviations.
S100 or GFAP staining showed the presence of glial cells within the small-bowel myenteric
plexuses in all three groups ([Fig. 4D–H]). The mean S100 expression level on the 3-point scale was 2 in all three groups
([Fig. 4D–F]). In contrast, the mean GFAP expression level on the 3-point scale was higher in
the gastroschisis group than in the sham and control groups (1.8 vs. 0.7 and 0.6,
respectively; p < 0.002) ([Fig. 4G–I]).
Interstitial Cells of Cajal Network
Immunostaining with antibody to c-kit identified ICCs between the circular and longitudinal
muscular layers of small-bowel specimens in all three groups ([Fig. 5]). However, when compared with sham and control groups, ICCs were less abundant in
eviscerated small bowel in the gastroschisis group and often exhibited weak c-kit
staining or an abnormally round shape without branches ([Figs. 2E] and [5]).
Figure 5 Interstitial cells of Cajal: c-kit immunostaining, magnification x40.
Discussion
In this rat model of gastroschisis, we showed that extrusion of the small bowel out
of the abdominal cavity during embryonic development led to peel formation, thickening
of all the small bowel wall layers except villi and impaired organization of the myenteric
plexus, and ICC network. These results contribute to the understanding of the intestinal
dysmotility seen in neonates with gastroschisis.
Improved understanding of the mechanisms involved in small-bowel damage in gastroschisis
is a prerequisite to the development of effective treatments. Most neonates with gastroschisis
do not require small-bowel resection and, therefore, few human intestinal specimens
are available for study; furthermore, resection is performed in humans only when the
bowel exhibits severe ischemia, which confounds the results of histological studies.
Therefore, investigations into the mechanisms underlying dysmotility in gastroschisis
rely essentially on animal models. The animal model used in our study replicates several
important features of human gastroschisis: small bowel exits the abdominal cavity
through the surgical incision in the abdominal wall and is therefore exposed to the
amniotic fluid; and the eviscerated bowel exhibits variable degrees of peel formation
with thickening, edema, and retraction. However, the exposure to amniotic fluid was
shorter than in human fetuses with gastroschisis, even after normalization for length
of gestation. Furthermore, bowel ischemia related to small-bowel compression through
a small defect may occur in human fetuses, as suggested both by clinical cases with
overt strangulation and by experimental studies.[8]
[9] In our study, in contrast, we created a large abdominal defect to avoid strangulation
and ischemia of the herniated bowel loops that would have compromised the analysis.
In our experimental study, we have replicated several typical features of human gastroschisis.
Also, our findings agree with those of the other studies using various models of gastroschisis
in rats,[7]
[10]
[11]
[12] lambs,[8] rabbits,[13] and chicks[14]: in the fetuses with gastroschisis, we found peel formation by gross examination
in 36% of the cases, significantly higher values for the IW/L ratio and weight score
compared with the sham and control fetuses, thickening of the serosa, muscular layers,
and submucosa but normal shape and thickness of the villi.
As intestinal dysmotility is a major clinical feature of human neonates with gastroschisis,
we investigated whether enteric nervous system or ICC network were affected in gastroschisis.
We conducted a detailed histological and immunohistochemical study of the myenteric
plexus and ICC network. We chose a qualitative method that highlights several dimensions
of the enteric nervous system development. We performed a qualitative analysis of
the myenteric plexus using morphotype comparison with specimen harvested from time-dated
E16 to E22 control fetuses. We first analyzed the organization of the myenteric plexus
in control fetuses during embryonic development. At E16, the myenteric plexus is organized
as a continuous belt located between both smooth muscle layers on a transversal small
bowel tissue section ([Fig. 1A]). From E17 to E19, a partition of this continuous belt occurred and the myenteric
plexus took an ovoid shape ([Fig. 1B–D]). From E20 to E22, the myenteric plexus took an increasing rounded shape, with enlarged
interplexus distance ([Fig. 1E], [F] and [Fig. 3A, B]). Similar maturation of the myenteric plexus during development has been previously
described in rats and human fetuses.[15]
[16] In our study, we compared E22 morphology of myenteric plexus from gastroschisis
fetuses to control and sham fetuses, and the maturity of myenteric plexus was graded
for each fetus from 1 (mature) to 3 (very immature). Although the myenteric plexus
was visible in the eviscerated small bowel from gastroschisis fetuses, the structure
of the plexus was consistently modified ([Fig. 3C–F]). The myenteric plexus was either immature (grade 2, 55%) or very immature (grade
3, 45%) compared with control and sham fetuses.
Immunohistochemistry with neuronal and glial markers, as well as ICC markers, allowed
us to analyze further the enteric nervous system phenotype. Our study showed glial
cells stained with S100 protein and GFAP antibody and enteric neuronal precursors
stained with Hoxb5 in all three groups of fetuses. Hoxb5 expression was not significantly
different in the eviscerated small bowel of gastroschisis fetuses from the intra-abdominal
small bowel in the sham and control fetuses suggesting that neural cells are present
in gastroschisis. However, previous studies in the same rat model found decreased
expression of other neuronal markers (synaptophysin, α-internexin, and neurofilaments)
in myenteric plexuses from the fetuses with gastroschisis,[10]
[11] a finding that might indicate impaired neuronal function or phenotype.
Although expression of S100, a glial cell marker, was normal in the eviscerated bowel,
the master glial-cell marker GFAP, however, was overexpressed. The glial cells ensheath
the neurons within the ganglionated plexus, to insure normal function of the enteric
nervous system by regulating the supply of substrate to the neurons.[17] Glial cells are also involved in scavenging neurotransmitters from the extracellular
space and produce cytokines.[17] Healthy intestinal specimens from patients with Crohn's disease exhibited disruption
of the glial-cell network, suggesting a role for glial cells in human inflammatory
bowel disease.[18] Further support for such a role was obtained in a study showing that fulminant enterocolitis
developed in transgenic mice devoid of enteric glia.[19] We cannot determine from our data whether the GFAP overexpression in eviscerated
bowel was related to increased glial cell proliferation, glial hyperplasia, increased
GFAP-gene expression, or post-transcriptional modifications affecting antibody binding.
However, the similar S100 expression across study groups suggested that the GFAP overexpression
in the gastroschisis group was perhaps related to functional modifications in glial
cell physiology. Consistent with our results, other studies have shown that GFAP expression
in central nervous system glia is altered by astrocyte injury and inflammation, and
GFAP overexpression in the enteric nervous system has been reported in bowel inflammation.[17]
[18] Also, increased expression of GFAP has been observed above the defect in human with
intestinal atresia, suggesting that obstruction might be a contributing factor.[15] Whether GFAP overexpression reflects a protective or a detrimental glial-cells response
to inflammation and/or obstruction in our gastroschisis model is however unknown.
Normal intestinal motility requires normal generation and propagation of electrical
slow waves throughout the smooth muscle layers.[6] ICCs serve as an electrical pacemaker coupled with the smooth muscle cells via low-resistance
electrical junctions.[6] Small bowel lacking ICCs does not actively propagate slow waves and exhibits blunted
responses to cholinergic and nitrergic enteric motor-neuron stimulation.[6] ICCs were evaluated with specific c-kit immunostaining on small bowel specimen from
all groups. In our study, ICCs in small bowel from fetuses with gastroschisis were
sparse and showed weak c-kit staining or an abnormally round shape without branches,
compared with the sham and control fetuses. Our findings are consistent with results
from several animal models of gastroschisis.[12]
[14]
[20]
[21] Marked intestinal dysmotility is seen in most human neonates with gastroschisis
suggesting impaired development of the enteric nervous system or ICC network. Our
findings support this clinical observation. Further support for this cause of delayed
intestinal motility comes from a clinical case that showed delayed maturation of the
ICC network and enteric neurons at birth in a specimen of normal-looking small bowel
from a human neonate with gastroschisis who required resection because of bowel ischemia.[22] Interestingly, examination of a second specimen taken 1 month later during surgery
to restore continuity showed improved maturation of the ICC network and enteric neurons.[22] Similarly, decreased expression of c-kit has been consistently observed in the proximal
segment of small bowel with intestinal atresia in a large series of 23 patients[15] Impairment of ICC morphology and network in our experimental gastroschisis study
may thus be secondary to functional obstruction.
Conclusion
In this rat model, we were able to replicate the intestinal lesions observed in human
gastroschisis, as attested by peel formation and parietal thickening of the eviscerated
small bowel. We observed several phenotypic alterations of myenteric plexus, glial
cells and ICC in the eviscerated small bowel that may be consistent with delayed maturation
of the myenteric plexus, and ICC network. These features may explain the intestinal
motility disorders observed in neonates with gastroschisis and give potential leads
for targeted therapies.