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Planta Med 2013; 79(02): 110-115
DOI: 10.1055/s-0032-1328068
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Silibinin In Vitro Protects A549 Cells from Staphylococcus aureus-Mediated Injury and In Vivo Alleviates the Lung Injury of Staphylococcal Pneumonia

Xin Wang   *
1   College of Quartermaster Technology, Jilin University, Changchun, PR China
,
Jing Dong   *
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Xiaohan Dai
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Yu Zhang
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Jianfeng Wang
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Hongen Li
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Chongjian Lu
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Wei Tan
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Xiaohan Gao
1   College of Quartermaster Technology, Jilin University, Changchun, PR China
,
Xuming Deng
2   Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China
,
Shijin Bu
3   College of Veterinary Medicine, Yangzhou University, Yangzhou, PR China
,
Xiaodi Niu
1   College of Quartermaster Technology, Jilin University, Changchun, PR China
› Author Affiliations
Further Information

Publication History

received 05 July 2012
revised 13 November 2012

accepted 18 November 2012

Publication Date:
08 January 2013 (online)

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Abstract

In this study, hemolysis, Western blot, and real-time RT-PCR assays were performed to evaluate silibininʼs activity against S. aureus α-toxin secretion. In addition, live/dead cell staining and lactate dehydrogenase activity assays were introduced to examine the influence of silibinin on α-toxin-induced cell injury in human alveolar epithelial cells. Furthermore, we tested the influence of silibinin on S. aureus pneumonia in a mouse model. We show that silibinin inhibits the expression of α-toxin in a dose-dependent manner and alleviates α-toxin-induced lung cell injury. The IC50 of silibinin that inhibits the hemolytic activity of S. aureus was 14.33 µg/mL for strain 8325–4. Moreover, this compound provides effective protection on the lung injury of staphylococcal pneumonia.

Key words

Staphylococcus aureus - α-toxin - silibinin - Silybum marianum - Asteraceae - A549 cells - pneumonia

* Both authors contributed equally to this work.