Alkamides from Echinacea angustifolia Interact with P-Glycoprotein of Primary Brain Capillary Endothelial Cells Isolated from Porcine Brain Blood Vessels

Anne Mahringer; Karin Ardjomand-Woelkart; Rudolf Bauer; Gert Fricker; Thomas Efferth

doi:10.1055/s-0032-1328090

Planta Medica, Table of Contents

Planta Med 2013; 79(03/04): 214-218
DOI: 10.1055/s-0032-1328090

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Alkamides from Echinacea angustifolia Interact with P-Glycoprotein of Primary Brain Capillary Endothelial Cells Isolated from Porcine Brain Blood Vessels

Anne Mahringer^*

¹Institute of Pharmacy and Molecular Biotechnology, Department of Pharmaceutical Technology and Pharmacology, University of Heidelberg, Heidelberg, Germany

,

Karin Ardjomand-Woelkart^*

²Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Karl-Franzens-University Graz, Graz, Austria

,

Rudolf Bauer

²Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Karl-Franzens-University Graz, Graz, Austria

,

Gert Fricker

¹Institute of Pharmacy and Molecular Biotechnology, Department of Pharmaceutical Technology and Pharmacology, University of Heidelberg, Heidelberg, Germany

,

Thomas Efferth

³Institute of Pharmacy and Biochemistry, Department of Pharmaceutical Biology, Johannes Gutenberg University, Mainz, Germany

› Author Affiliations

Abstract

The blood-brain barrier prevents the passage of toxic compounds from blood circulation into brain tissue. Unfortunately, drugs for the treatment of neurodegenerative diseases, brain tumors, and other diseases also do not cross the blood-brain barrier. In the present investigation, we used isolated porcine brain capillary endothelial cells and a flow cytometric calcein-AM assay to analyze inhibition of P-glycoprotein, a major constituent of the blood-brain barrier. We tested 8 alkamides isolated from Echinacea angustifolia and found that four of them inhibited P-glycoprotein-mediated calcein transport in porcine brain capillary endothelial cells.

Key words

ABC transporter - alkamides - blood-brain barrier - Echinacea angustifolia - Asteraceae - P-glycoprotein - inhibitor

Full Text

References

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