Abstract
Together with twelve known compounds (2–13), melodamide A (1), a new phenolic amide possessing p-quinol moiety, was purified and characterized from the methanolic extracts of the
leaves of Melodorum fruticosum. The structure of melodamide A (1) was established with a combination of 2D NMR experiments, HR-ESI-MS and X-ray analyses.
The other known compounds were identified by comparison of their spectroscopic and
physical data with those reported in the literature. Moreover, some isolated compounds
were examined for their inhibitory activity towards superoxide anion generation and
elastase release in human neutrophils. Among the tested compounds, 1, 3, and 5 exhibited strong inhibition of superoxide anion generation with IC50 values ranging from 5.25 to 8.65 µM. Furthermore, synthesis and biological evaluation
of melodamide A (1) and its analogs (14a–p) were described.
Key words
Melodorum fruticosum
- Annonaceae - melodamide A - superoxide anion generation - elastase release - anti-inflammatory