Cancer Preventive and Curative Attributes of Plants of the Cactaceae Family: A Review

Key words

Cactaceae - cactus - chemoprevention - anticancer effects - antioxidant - apoptosis

Abbreviations

AFB₁ : aflatoxin B₁

B[a]P: benzo[a]pyrene

CDDP: cis-diamine dichloroplatinum

CME: cactus mixture extract

CTX: cyclophosphamide

Cyt. c : cytochrome c

DMBA: 7,12-dimethylbenz[a]anthracene

4-HPR: N-(4-hydroxyphernyl) retinamide

HSP27: heat shock protein 27

HSP70: heat shock protein 70

i. p.: intraperitoneal

MDA: malondialdehyde

NF-κB: nuclear factor-kappaB

NO: nitrogen monoxide

PARP: poly (ADP) ribose polymerase

PC: protein carbonyls

RBC-CaR: RBC-cancer cell immune adherence

RFER: RBC immune adherence enhance factor

RFIR: RBC immune adherence inhibitor factor

ROS: reactive oxygen species

SOD: superoxide dismutase

TPA: 12-O-tetradecanoylphorbol-13-acetate

VEGF: vascular endothelial growth factor

Introduction

Ample evidence exists of the steadily increasing occurrence of cancer in Western societies being the result of environmental factors and of lifestyle [1]. For example, a recently published article suggests that cancer is practically a man-made disease, steadily growing since the beginning of the industrial revolution in the mid-19th century but rarely having been witnessed in the ancient world or in current non-modern (frequently referred to as “primitive”) societies [2]. Targeting both lifestyle and environment, thus adopting a preventive approach, seems to be the preferred and most logical way to cope with the problem. However, current cancer research focuses mainly on curing the disease, while it should preferably be based on developing drugs of plant origin because, unlike their synthetic chemical counterparts, their predisposition to produce severe side effects is small.

Plants of the Cactaceae family are mostly desert and semidesert habitants, which, owing to harsh environmental stress conditions (water scarcity, strong radiation, temperature differences, and poor soil) are conceived to have developed highly effective defense systems, allowing them to successfully cope with the environment. These defense systems are made of phytochemicals, such as alkaloids, flavonoids, terpenes, and tannins, already shown to exhibit remarkable bioactivities against human diseases such as cancer [3], [4] and diabetes [5].

The fact that herbs and plant-derived products lack much of the toxicity present in synthetic chemicals enhances their appeal for treating cancer and for long-term preventive strategies. Cactus pears and cladodes (modified stems) contain, among other components, pectin, carotenes, betalains, ascorbic acid, and quercetin derivatives, all of which are known to possess antioxidant properties, marking them a potential source for anticancer and cancer preventive drugs. Emerging studies indicate remarkable anticancer activities displayed by cactus pear extracts. Deprived of toxic effects, cactus-derived components can be easily used, for example, as dietary supplements in normal and high-risk populations for cancer [6]. Cactus pears have been used by Native Americans for centuries as a dietary supplement, and these ethnic groups show lower cancer rates when compared to white and African Americans [7], [8], [9]. This historical fact is further confirmed by experimental results, such as shown in this review, indicating the potential of plants of the Cactaceae family for inhibiting the growth of various cancer cells and inducing anticancer biological effects in vivo.

The aim of this article is to review the experimental work performed to date on plants of the Cactaceae family in cancer research. To the best of our knowledge this attempt is the first of its kind. Various pure compounds isolated from cacti are presented in [Table 1]. The anticancer activities of these compounds as well as of extracts from plants belonging to the Cactaceae family are exhibited in vitro ([Table 2]) and in vivo ([Table 3]). [Fig. 1] displays pictures of cacti endowed with chemopreventive and anticancer properties.

Table 1 Structures of anticancer compounds derived from plants of the Cactaceae family.

Compound	Chemical class	Plant source	Reference
([Fig. T 1])	Betalains	O. ficus-indica	Sreekanth et al., 2007 [16]
([Fig. T 2])	Triterpenoids	S. stellatus	Kinoshita et al., 1999 [38]
([Fig. T 3])	Terpenes	P. bleo	Malek et al., 2009 [28]
([Fig. T 4])	Substituted phenols	P. bleo; P. grandifolia	Malek et al., 2009 [28]; Sri Nurestri et al., 2009 [30]
([Fig. T 5])	Sterols	M. geometrizans	Salazar et al., 2011 [36]
([Fig. T 6])	Diterpene alcohols	P. bleo	Malek et al., 2009 [28]
([Fig. T 7])	Substituted phenols	P. bleo	Malek et al., 2009 [28]

Fig. T 1

Fig. T 2

Fig. T 3

Fig. T 4

Fig. T 5

Fig. T 6

Fig. T 7

Table 2 In vitro anticancer effects of extracts derived from and components included in plants of the Cactaceae family.

Cactus	Fraction/component studied	Cellular effect	Mechanism	IC50	Reference
O. ficus-indica O. robusta Amarillo O. robusta Gavia O. robusta Tapon O. streptacantha Cardon O. violaceae Moradillo O. rastrera Rastrero O. leucotricha Duraznillo Blanco O. leucotricha Duraznillo Rojo	Prickly pear juice	Diminished the viability of prostate, colon, mammary, and hepatic cancer cells	Antioxidant effects		Chavez-Santoscoy et al., 2009 [13]
O. ficus-indica	Aqueous cactus pear extract	Inhibited the growth of TCL-1, HeLa, Me180, IOSE, SKOV3, OVCA420, UM-UC6, T24, and UM-UC9 cells	↑G1 phase; ↓G2 & S phases		Zou et al., 2005 [6]
		Displayed growth-inhibitory effect in OVCA420 and SKOV3 cells	↑Apoptosis; ↑Bax; ↓Bcl2, ↑caspase-3; ↓NF-κB, ↓p-/SAPK/JNK; ↑p53; ↑p21; ↑p-AKT		Feugang et al., 2010 [14]
	Betanin	Exhibited antiproliferative effects on in K562 cells	↑Apoptosis; ↓Bcl-2; ↑caspase; ↑Cyt. c; ↑PARP cleavage	40 µM	Sreekanth et al., 2007 [[16]
O. humifusa	Aqueous fruit extract	Inhibited the proliferation of MCF-7 cells	Induced G1 arrest		Yoon et al., 2009 [17]
	Fruit and stem aqueous extracts	Suppressed the growth of U87MG cells	↑Apoptosis; ROS-induced G1 arrest		Hahm et al., 2010 [18]
P. bleo (Kunth) DC	Aqueous leaf extract	Exhibited cytotoxic effects in T-47D cells	↑Apoptosis; ↑caspase-3; ↑c-myc	2.0 µg/mL (EC50)	Tan et al., 2005 [24]
		Inhibited the proliferation of 4 T1 cells	↑Apoptosis		Er et al., 2007 [26]
	Methanolic and ethyl acetate extracts	Induced cytotoxicity against KB cells		4.5–6.5 µg/mL	Sri Nurestri et al., 2008 [27]
	Dihydroactinidiolide	Induced cytotoxicity against HCT116 cells		5.0 µg/mL	Malek et al., 2009 [28]
	2,4-Di-tert-butylphenol	Induced cytotoxicity against KB, CasKi, A546, and MCF-7 cells		0.81–6 µg/mL
	Phytol	Induced cytotoxicity against KB cells		7.1 µg/mL
	α-Tocopherol	Induced cytotoxicity against CasKi, A549, and MCF-7 cells		6–7.5 µg/mL
P. grandifolia Haw	Hexane extract	Induced cytotoxicity against KB cells		5 µg/mL	Sri Nurestri et al., 2009 [30]
	Ethyl acetate extracts	Induced cytotoxicity against KB and MCF-7 cells		16–20 µg/mL
	2,4-di-tert-butylphenol	Exerted cytotoxicity against KB cells		0.81 µg/mL
	Methanolic extract of leaves	Showed cytotoxicity against Saos-2 cells			Liew et al., 2012 [31]
H. undatus; H. polyrhizus	Methanolic extracts of peel and flesh	Displayed antiproliferative effects against AGS and MCF-7 cells			Kim et al., 2011 [32]
		Exhibited growth-suppressive effects of B16F10 cells			Wu et al., 2006 [33]
	Methanolic extract of fruits	Inhibited the proliferation of MCF-7 cells	Nitric oxide scavenging		Jayakumar et al., 2011 [34]
M. geometrizans	Peniocerol; macdougallin	Induced cytotoxicity against PC-3, K-562, U-251, MCF-7, and HCT-15 cells		7.55–24.73 µM	Salazar et al., 2011 [36]
S. stellatus Riccob.	Betulinic acid; 3-O-acetylbetulinic acid	Exerted antitumorigenic activity against HeLa cells			Kinoshita et al., 1999 [38]
Non-identified cactus	Polysaccharide	Inhibited the growth of SK-MES-1 cells			Wu et al., 2012 [44]

Table 3 In vivo anticancer effects of extracts derived from and components included in plants of the Cactaceae family.

Cactus	Fraction/component studied	Biological effects	Mechanism	Dose (duration)/route	Reference
O. ficus-indica	Aqueous cladode extract	Protected against B[a]P-induced genotoxicity in BALB/c male mice	↓MDA; ↓catalase; ↓HSP70; ↓HSP27; ↑p53, ↑Bax; ↓Bcl-2	50 mg/kg (15–30 days); i. p.	Brahmi et al., 2011 [20]
		Reversed AFB1-induced hepatic damage in BALB/c male mice	↓MDA; ↓PC; ↓HSP70; ↓HSP27; ↓p53; ↓Bax; ↑Bcl-2	50 mg/kg (15–30 days); i. p.	Brahmi et al., 2011 [21]
		Exhibited antigenotoxic effects in CDDP-exposed male BALB/c mice	↓MDA; ↓catalase; ↑SOD;↓p53; ↓Bax; ↑Bcl-2	50 mg/kg (15–30 days); i. p.	Brahmi et al., 2012 [22]
	Aqueous cactus pear extract	Suppressed tumor growth in female BALB/c mice xenografted with SKOV3 cells	↑Apoptosis; ↓VEGF	0.4 mL/mouse/day (5 days); i. p.	Zou et al., 2005 [6]
O. humifusa	Cactus fruit powder	Decreased numbers of papillomas and epidermal hyperplasia in DMBA-treated female BALB/c mice	↑Apoptosis; ↓MDA; ↑SOD; ↑GST	1, 3 % (3 weeks); diet	Lee et al., 2012 [23]
Non-identified cactus	Polysaccharides from cactus pear	Inhibited the growth of S180 transplanted tumors in mice	↑Apoptosis; ↓MDA; ↑SOD; ↓NO	Non-specified	Liang et al., 2008 [45]
		Suppressed the growth of S180 carcinoma and prolonged the survival of H22 tumor-bearing mice	Non-specified	Non-specified	Ji et al., 2004 [46]
		Induced antitumor effect on S180 tumor-bearing mice	↑RBC-CaR; ↑RFER; ↓RFIR; ↑sialic acid	Non-specified	Ji et al., 2005 [47]

Fig. 1 Pictures of plants of the Cactaceae family exhibiting anticancer properties: A O. ficus-indica, B O. humifusa, C H. ubdatus, D H. polyrhizus, E M. geometizans, F S. thurberi, G S. stellatus, and H C. peruvianes (C. quadrangularis). (Color figure available online only.)

Search Methodology

The purpose of this review has been to provide the interested reader with a broad view of the research work performed up-to-date on the subject. To achieve this goal, the scientific search engine “SciFinder” was found to be an extremely useful tool, as it retrieves information from both MEDLINE and CAPLUS data bases. In some cases, the original articles were obtained and carefully examined. In other cases, only the abstracts have been used. As the amount of work performed on the subject is not very large, we have tried our best to incorporate into this article any work located in the scientific literature adding new information.

Antitumor effects of extracts and pure components derived from cacti

Genus Opuntia

Opuntia, a genus of the Cactaceae family, includes about 200 species and is comprised solely of prickly pear cacti. The stems of these perennial cacti are composed of flattened segments, intensely green and covered in bristles and spines according to the variety. The flowers are bright yellow, cream, or gold in color and are found along the margins of most mature upper segments. The petals of the flowers have a waxy texture, and sometimes the centers and sepals of the flowers are reddish in color. The fruits vary greatly in taste, size, and edibility, developing in color from green to red, pink, and orange; they are three to five centimeters long and full of small marble-like seeds. The plant thrives in full sun.

The best known species of this genus is the xerophyte cactus Opuntia ficus-indica (L.) Mill. ([Fig. 1 A]), commonly called “Indian fig”, which attracts significant interest as a nutritional and pharmacological power source. This tree-like cactus is widespread throughout Central and South America, Australia, South Africa, and the whole Mediterranean area [10]. The great number of potentially active nutrients and their multifunctional properties make the juice of the cactus pear (the fruit of O. ficus-indica, also referred to as “prickly pear”) a perfect candidate for the production of health-promoting food and food supplements. Health benefits and medicinal and nutritional use of the cactus pear, including reduction in the risk of cancer, were reviewed [6], [11], [12].

In vitro studies: Juices extracted from nine prickly pears (belonging to genus Opuntia) were found in vitro to diminish the viability of prostate, colon, mammary, and hepatic cancer cells without affecting normal fibroblast viability ([Table 2]). The differences in anticancer effects among the tested juices were attributed to variations in their content of phytochemicals, such as flavonoids and betalains, compounds known to prevent oxidative stress and cancer [13].

Aqueous extract of the Arizona cactus pear were used by Zou et al. [6] to treat immortalized ovarian and cervical epithelial cells, as well as ovarian, cervical, and bladder cancer cells. Cells exposed to Arizona cactus aqueous pear extracts exhibited a significant increase in apoptosis and growth inhibition in both immortalized epithelial cells and cancer cells in a dose- and time-dependent manner. It also affected the cell cycle of cancer cells by increasing G1 and decreasing G2 and S phases.

An aqueous CME derived from the Arizona cactus pear reduced the growth of ovarian cancer cells by inducing apoptosis in vitro. Treating normal, immortalized ovarian and ovarian cancer cells (OVCA420 and SKOV3, respectively) with 5 and 10 % CME exhibited a dramatic increase of ROS. Greater levels of DNA fragmentation, together with a perturbed expression of apoptotic-related genes, namely Bax, Bad, caspase-3, Bcl-2, p53, and p21 and ROS-sensitive genes, such as NF-κB and c-jun/c-fos, were observed in the treated cancer cells, and the NF-κB and p-SAPK/JNK expressions were decreased after three days of treatment. The CME significantly induced apoptosis in cancer cells, attributed to the accumulation of intracellular ROS, which may activate a cascade of reactions leading to apoptosis [14].

Betalains are water-soluble nitrogenous vacuolar pigments present in flowers and fruits of many caryophyllales with potent antioxidant, anti-inflammatory, and anticarcinogenic properties [15]. Betanin ([Table 1]), the most abundant phytochemical of all betalains, isolated from the fruits of O. ficus-indica, was found to decrease dose- and time-dependent proliferation of K562 human chronic myeloid leukemia cells. The results also indicated that betanin induces apoptosis in K562 cells through alteration of mitochondrial membrane integrity, leading to Cyt. c leakage from mitochondria into the cytosol, PARP cleavage, downregulation of Bcl-2, and reduction in the membrane potential [16].

Opuntia humifusa (Raf.) Raf. ([Fig. 1 B]), commonly known as the Eastern prickly pear or Indian fig, is a native cactus found in most of eastern North America. It is also widely distributed in the southern regions of the Korean peninsula and known to have bioactive functions and medicinal benefits in the treatment of various diseases such as arteriosclerosis, diabetes mellitus, gastritis, and hyperglycemia. A study investigated total polyphenol and flavonoid contents of the plantʼs fruit and its anticarcinogenic effects on human breast cancer. Water extracts of the fruit of O. humifusa were found to inhibit MCF-7 human breast cancer cell proliferation and to induce G1 arrest [17].

Hexane and ethyl acetate water partitioned extracts of the fruits and stems of O. humifusa were tested on U87MG human glioblastoma cells and found to induce both apoptosis and G1 arrest. The number of viable U87MG cells decreased in a concentration-dependent manner following the extract treatment [18].

Three Opuntia polysaccharides exhibited remarkable concentration-dependent inhibitory effects on various human cancer cells. The results showed that the medicinal cactus has the best inhibitory effect on ANIP human lung adenocarcinoma cells, the edible cactus has the best inhibitory effect on K562 human chronic myeloid leukemia cells, and another cactus has the best inhibitory effect on HeLa cervical carcinoma cells [19].

In vivo studies: The antiproliferative efficacy of O. ficus-indica was tested against B[a]P, a widespread environmental genotoxin classified as probably carcinogenic to humans. The aim of the study was to investigate the in vivo protective effect of an extract obtained from O. ficus-indica cladode against B[a]P using Balb/c mice. The extract exhibited total reduction of B[a]P-induced oxidative damage for all tested markers. It caused apoptosis via inhibition of antiapoptotic proteins Bcl-2 expression and the induction of p53 and Bax expression, thus modulating the p53-dependent apoptotic pathway to restrict the B[a]P toxicity ([Table 3]) [20]. The same investigators also showed that the cladode extract of O. ficus-indica induced total reduction of AFB₁-induced genotoxicity in mice. The hepatoprotective effect of the extract against aflatoxicosis in mice was attributed to the promotion of the antioxidant defensive system [21]. In an extension of the aforementioned studies, the same extract has been shown to be beneficial in reversing CDDP-induced kidney dysfunction in mice through its antioxidant and antiapoptotic activities [22].

The effect of cactus pear solution on inhibiting tumor growth in mice indicated by tumor size was compared with a synthetic retinoid, 4-HPR, a compound currently being used as a chemopreventive agent in ovarian, cervical, and bladder cancer clinical trials. The inhibitory effect of 4-HPR was found not to be significantly different than that induced by the cactus pear extract solution. The cactus pear extract significantly suppressed ovarian tumor growth in nude mice, increased annexin IV expression (indication of apoptosis) and decreased VEGF expression [6].

O. humifusa was investigated for its in vivo chemopreventive effect on skin carcinogenesis induced by DMBA and TPA in mice. Significant decrease in the numbers of papilloma and epidermal hyperplasia was observed in mice fed with O. humifusa, compared to the control group. The chemopreventive effects of O. humifusa on chemical carcinogenesis in the mouse skin are thought to be associated with the reduction of oxidative stress via the modulation of cutaneous lipid peroxidation, enhancement of the total antioxidant capacity, especially in the phase II detoxifying enzyme, and a partial apoptotic influence [23].

Genus Pereskia

Pereskia is a genus of about 25 species that do not resemble most other cacti as they have shapely privet-like leaves and thin stems. Their native range are the areas between Mexico and Brazil, often dry forest or thorny scrubs. Some species are epiphytes. However, all of them have cactus-like flowers despite the fact that some species of the Pereskia genus do not resemble cacti or succulents. Pereskia bleo (Kunth) DC, commonly known as “Pokok Jarum Tujuh Bilah” (in Malay) and “Cak-Sing Cam” (in Chinese) by the locals, is a leafy and spiny shrub known to have many medicinal properties and has been used as a natural remedy in cancer-related diseases, either eaten raw or taken as a concoction brewed from fresh plants. It is believed to have anticancer, antitumor, antirheumatic, antiulcer, and anti-inflammatory properties and has been used as a remedy for the relief of headache, gastric pain, ulcers, hemorrhoids, atopic dermatitis, and for revitalizing the body [24]. The leaf of this plant has been used in Malaysian traditional medicine for the prevention and treatment of breast cancer [25].

In vitro studies: The methanol extract of P. bleo indicated in vitro cytotoxic activity against T-47D breast carcinoma cells with an EC₅₀ of 2.0 µg/mL. T-47D cell death elicited by the extract was attributed to DNA fragmentation, a hallmark of apoptosis. Ultra-structural analysis also revealed apoptotic characteristics in the extract-treated cells. RT-PCR analysis indicated increased mRNA expression levels of c-myc and caspase-3 in cells treated with the extract. However, p53 expression was only slightly increased as compared to caspase-3 and c-myc. The results suggested that the methanol extract of P. bleo may contain bioactive compound(s) causing T-47D breast carcinoma cell death by apoptotic mechanism via the activation of caspase 3 and c-myc pathways [24].

Er et al. [26] found that an aqueous extract from the leaves of P. bleo induced a significant antiproliferative activity in a mouse mammary cancer cell line (4 T1) and in a normal mouse fibroblast cell line (NIH/3 T3). An upward trend of apoptosis was observed in both 4 T1 and NIH/3 T3 cells treated with increasing concentrations of the aqueous extracts, and the level of apoptosis observed at all the concentrations of the extract tested was consistently higher than necrosis.

The crude methanol extract of P. bleo and its fractionated ethyl acetate extract were found to possess a notably high cytotoxic effect against human nasopharyngeal epidermoid (KB) cells with IC₅₀ values of 6.5 and 4.5 µg/mL, respectively. Four compounds isolated from the ethyl acetate fraction of P. bleo were identified as phytol, β-sitosterol, 2,4-di-tert-butylphenol, and vitamin E [27]. In a later study, dihydroactinidiolide, 2,4-di-tert-butylphenol, α-tocopherol, and phytol were isolated, identified, and their cytotoxicity was tested against five human carcinoma cell lines, namely KB, CasKi, HCT 116, MCF7, and A549. This study confirmed that 2,4-di-tert-butylphenol, isolated from the active ethyl acetate fraction derived from the leaves of P. bleo, possessed remarkable cytotoxic activity against KB cells, with an IC₅₀ value of 0.81 µg/mL, while each of the other isolated components displayed cytotoxicity of around 6 µg/mL against KB, and roughly the same value against the other cell lines. Furthermore, unlike doxorubicin, a currently used cytostatic drug for the treatment of cancer, crude extracts and pure components derived from P. bleo have low toxicity against normal MRC-5 cells, indicating better selectivity than that of doxorubicin [28].

The leaves of P. grandifolia Haw., another Cactaceae plant of the genus Pereskia, commonly known as “Jarum Tujuh Bilah” in Malaysia, have been traditionally used as a natural remedy in folk medicine by the locals, and their high antioxidant potential was confirmed [29]. The hexane fraction of P. grandifolia induced a remarkable cytotoxic effect against KB cells with IC₅₀ values of 5.0 µg/mL, while the ethyl acetate fraction displayed a high cytotoxic effect against both KB and MCF-7 cells with IC₅₀ values of 16.0 and 20.0 µg/mL, respectively. 2,4-di-tert-butylphenol, isolated from the active ethyl acetate fraction of this plant, possessed very remarkable cytotoxic activity against KB cells, with an IC₅₀ value of 0.81 μg/mL [30].

Crude methanol extract of the leaves of P. grandifolia, a plant also used in traditional Chinese medicine, exhibited cytotoxicity against human Saos-2 osteosarcoma cells under normoxia or hypoxia. It was found that the relative cytotoxicity on the Saos-2 cells was different in hypoxic versus normoxic conditions [31].

Genus Hylocereus

Pitaya (family Cactaceae, subfamily Cactoideae), commonly known as “dragon fruit”, has generated considerable consumer interest because of its attractive color and micronutrient content. White-fleshed pitaya is considered a tropical vine cactus, and it is the most cultivated species in the cactus family. Originating in Central and South America, it is used as an ornamental and fruit crop and named for the wavy margins of its ribs. The stems are scandent, creeping, crawling, or clambering with many branches, joints, and ribs and visible aerial roots. This cactus produces a huge trumpet-like flower and a large colorful fruit with scales resembling those of an artichoke. The solid mass of white flesh inside the red or pink fruit is enlivened by small black seeds, and the fruit is generally thornless. This cactus is easily grown in warm areas, is sensitive to temperatures over 40 °C and below 10 °C, enjoys compost and ample water and grows well on walls, trees, or trellises.

In vitro studies: One study conducted by Kim et al. [32] investigated the total polyphenol and flavonoid content and antioxidant activity of extracts of the flesh and peel of white-fleshed and red-fleshed pitayas [Hylocereus undatus (Haworth) Britton & Rose, [Fig. 1 C] and Hylocereus polyrhizus (F. A. C. Weber) Britton & Rose [Fig. 1 D], respectively] collected from Jeju Island, Korea. Their antiproliferative effect on several cancer cell lines was also investigated. Methanol extracts of the peels of both pitayas showed antiproliferative activity against AGS human gastric and MCF-7 breast cancer cells stronger than exhibited by the flesh extracts. Positive correlation was found between peel and flesh content of polyphenols and flavonoids, and their respective antiproliferative activities. Negative correlations were found between percent cell viability of HeLa, AGS, and MCF-7, and the total polyphenol content. Wu and colleagues [33] showed that the peel of red-fleshed pitaya is a stronger inhibitor of the growth of B16F10 melanoma cancer cells than the flesh. In another study, NO-induced proliferating MCF-7 cells were treated with methanol extracts of cactus fruits. Chiku and “dragon fruit” extracts exhibited remarkable inhibition of cell proliferation. The results were attributed to the scavenging of the cell proliferation-inducing nitric oxide by phytochemicals included in the fruit extract, resulting in the inhibition of MCF-7 cell proliferation [34].

A patent discloses an anticancer pharmaceutical composition containing H. undatus extract capable of suppressing cancer cell proliferation and used for the prevention and treatment of cancer [35].

Genus Myrtillocactus

Myrtillocactus geometrizans (Mart. ex Pfeiff.) Console (Bilberry cactus, Whortleberry cactus, or Blue Candle, [Fig. 1 E]) is a species of cactus in the genus Myrtillocactus native to central and northern Mexico. It is a large, shrubby cactus and can grow to be 4–5 meters in height with candelabra-like branches. The stems are 6 to 12 cm in diameter with five or six ribs. The flowers are white, pale yellow, or cream colored, open in the morning, and are pollinated by bees and flies. It is a fast growing cactus, tolerant of poor soils and mild frosts and heat up to 45 °C. It does not grow well in saline soil or heavy shade. The fruit is a berry, 1 to 2 cm in diameter, resembling a blueberry.

In vitro studies: The sterols peniocerol and macdougallin isolated from M. geometrizans showed cytotoxicity against several human cancer cell lines, including PC-3 human prostate carcinoma, K-562 leukemia, U-251 central nervous system carcinoma, MCF-7 breast carcinoma, and HCT-15 colon carcinoma. Peniocerol and macdougallin displayed moderate cytotoxicity against all cancer cell lines with IC₅₀ values between 7.55 and 24.73 µM. Peniocerol was more active than macdougallin against all the human cancer lines tested, except K-562 cells, against which macdougallin showed higher activity than peniocerol. The IC₅₀ values were found to be an order of magnitude higher than those exhibited by doxorubicin [36].

Genus Stenocereus

Stenocereus is a genus of columnar or tree-like cacti. Stenocereus thurberi ssp. littoralis (K.Brandegee) N. P.Taylor (also called organ pipe cactus, [Fig. 1 F]) is native to Mexico and the southwestern United States. It is found in rocky desert areas. This cactus species has several narrow stems that rise vertically, growing from a single trunk. The stem can be 15 to 20 cm thick and grows to a height of five meters, usually with no branches, giving the plant its nick-name. Mature plants produce funnel-shaped white flowers that open at night and close during the day, usually pink or white in color with reddish sepals. The flowers are pollinated by bats, wild bees, and flies. The fruit is a round, thorn-covered ball, very sweet with numerous black seeds inside the crimson flesh. The plant likes well drained soil and is sensitive to frost, being usually planted as a dry garden fruit or by a southern wall.

S. thurberi has profound influences on skin cancers and lesions. Though most of the evidence is anecdotal at this time, this is a line of research worth pursuing. Yetman [37] described the folkloric medicinal uses of the plant: “the fleshy moist stem is singed to remove spines then applied directly to the flesh for snake and insect bites, a remedy that I tested with positive results when an assassin bug bit my colleague”. The plant is known to be a healing one in Mexico, and it is the market place cure for skin cancers and topical wounds of all kinds.

In vitro studies: Seventeen triterpenes isolated from cacti and 10 derivatives thereof were examined for the in vitro inhibition of tumor-promoting effects, such as the stimulation of ³²Pi incorporation into phospholipids of cultured cells. The inhibitory potency of betulinic acid, extracted from Stenocereus stellatus (Pfeiff.) Riccob. ([Fig. 1 G]), and that of its acetylated derivative were found to exceed by far those of the other compounds tested (42.2 % and 100 % at a dose of 5 µg/mL and 50 µg/mL, respectively) [38].

Genus Cereus

Cereus cacti are large tree-like columnar cacti with four to ten well-defined ribs, thick stems, large white flowers, floral tubes that are sometimes scaly, and tasty pink or red fruits that grow out of the rib margins. They are pollinated by bees, bats, and birds though the flowers are fully open only at night and close two to three hours after sunrise. The plants are able to endure temperatures from − 5 °C to 45 °C and are tolerant of many soil types. They are found in the southwestern United States, Mexico, and Central America. The Cereus family includes many species, some of which have been moved into other botanical families as more is learned about columnar cacti.

Cereus cacti are described as potential crop plants but are also mentioned as plants used to treat cancer, especially Cereus quadrangularis Haw. ([Fig. 1 H]) [39]. The latter is also mentioned by Karimi et al. [40], under its synonym Cereus peruvianus, as a medicinal plant effective in treating breast cancer among other diseases.

Non-specified cacti

In vitro studies: The polysaccharides of the cactus cladode consist of rhamnose, fructose, galactose, xylose, arabinose, mannose, and uronic acids, and have been confirmed to possess diverse biological activities, including anticancer efficacy. Guo [41] described the extraction, purification, structure, and bioactivity of polysaccharides from the cactus cladode. The biological effects of the polysaccharides, flavonoids, and alkaloids present were reported, with special emphasis on their role in the prevention and treatment of chronic diseases, including cancer [42].

An invention describes a process for extracting cactus juice and the application thereof in several oncologic diseases, which consists of selecting a cactus with special characteristics, sectioning the arms thereof in a transversal manner for extracting the white color pulp and then cooking the same, the pulp being subjected to a filtration process so as to be subsequently stored in containers and used as a treatment of different diseases [43].

The antitumor effect of wild cactus polysaccharide on in vitro cultivated SK-MES-1 lung squamous carcinoma cells was investigated. The lowest inhibition concentration and tumor inhibitive ratio of wild cactus polysaccharide to SK-MES-1 for 24 h and 48 h were 0.0625 mg/mL and 34.06 %, and 0.0625 mg/mL and 35.37 %, respectively [43].

In vivo studies: The antitumor effect of polysaccharides extracted from cactus pear fruit in S180 murine sarcoma-bearing mice was investigated. The extracted polysaccharides possess certain antitumor effects, which could induce apoptosis, increase antioxidation and promote immune responses [45].

A study investigated the antitumor effects of three kinds of cactus polysaccharides on mice bearing S180 carcinoma and H22 hepatocellular carcinoma. The results showed that the three polysaccharides have antitumor effects on S180 carcinoma and also a life lengthening effect on H22 hepatocellular carcinoma-bearing mice [46]. Another study showed that the cactus polysaccharides increased the content of RBC-CaR and RFER, decreased the content of RFIR and raised the sialic acid content. The cactus polysaccharides further improved the erythrocyte function of tumor-bearing mice, which was assumed to be one of the antitumor mechanisms [47]. Polysaccharides of a cactus increased microviscosity and decreased membrane lipid fluidity of the S180 cell membrane, leading to the conclusion that the polysaccharides of a cactus change the function of tumor cell signal transduction and communication to play a key role in antitumor effects [48].

Conclusion and Perspective

The gradually emerging trend of applying bioactive extracts and pure components of plant origin to treat and prevent cancer stems mainly from the almost unavoidable severe side effects involving the use of chemical drugs and their frequently low efficacy. The experimental works disclosed in this review indicate the value of plants of the Cactaceae family as potential sources for preventive and curative anticancer drugs.

Based on available literature as presented in our manuscript, studies conducted using various cancer cell lines have provided impressive evidence of anticancer activities. Only a limited number of studies have documented similar results using in vivo tumor models. Currently, very limited information is available on whether concentrations showing activity in vitro are reachable in the blood or serum of laboratory animals or not. As a matter of fact, the same plant or bioactive component has not been studied in both in vitro and in vivo systems. While this represents a limitation to our existing knowledge on the anticancer potential of plants belonging to the Cactaceae family, it is expected that future studies will explore this area of research.

As this review shows, only a few plants out of the 1500 included in this family have been tested for their chemopreventive and anticancer effects, while the great bulk still remains unexplored. Also a very small number of pure components isolated from cacti were tested for their antineoplastic properties. In most cases, only crude extracts or crude pressed juices were tested either in vitro or in vivo. Somewhat more refined extraction procedures are mentioned only twice [18], [27]. Only one publication compares the anticancer activity of a crude extract derived from a cactus to that of a pure component isolated from it [28]. No mention is made of highly bioactive extract combinations or of clinical trials. Also, no mention was found of actual or possible side effects involving the use of these plants in cancer prevention and cancer therapy. We are in the opinion that the importance of this review resides in directing attention of the scientific community to this family of plants, which is on the one hand, highly potential in treating and preventing cancer, but on the other hand still almost unexplored.

Acknowledgements

The authors thank Ms. Robin Permut for manuscript editing and Anat Raz for providing cacti pictures.

• References

• 1 Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011; 61: 69-90
• 2 David AR, Zimmerman MR. Cancer: an old disease, a new disease or something in between?. Nat Rev Cancer 2010; 10: 728-733
• 3 Harlev E, Nevo E, Lansky EP, Lansky S, Bishayee A. Anticancer attributes of desert plants: a review. Anticancer Drugs 2012; 33: 255-271
• 4 Harlev E, Nevo E, Lansky EP, Ofir R, Bishayee A. Anticancer potential of aloes: antioxidant, antiproliferative and immunostimulatory attributes. Planta Med 2012; 78: 843-852
• 5 Harlev E, Nevo E, Mirsky N, Ofir R. Antidiabetic attributes of desert and steppic plants: a review. Planta Med 2013; 79: 425-436
• 6 Zou D, Brewer M, Garcia F, Feugang JM, Wang J, Zang R, Liu H, Zou C. Cactus pear: a natural product in cancer chemoprevention. Nutr J 2005; 4: 25
• 7 Knishinsky R. Prickly pear cactus medicine. Rochester: Healing Arts Press; 1971
• 8 Kay MA. Healing with plants in the American and Mexican West. Tucson: The University of Arizona Press; 1996
• 9 Cornett J. How Indians used desert plants. Palm Springs: Nature Trails Press; 2000
• 10 Galati EM, Mondello MR, Giuffrida D, Dugo G, Miceli N, Pergolizzi S, Taviano MF. Chemical characterization and biological effects of Sicilian Opuntia ficus indica (L.) Mill. fruit juice: antioxidant and antiulcerogenic activity. J Agric Food Chem 2003; 51: 4903-4908
• 11 Feugang JM, Konarski P, Zou D, Stintzing FC, Zou C. Nutritional and medicinal use of Cactus pear (Opuntia spp.) cladodes and fruits. Front Biosci 2006; 11: 2574-2589
• 12 El Gharras H. Cactus pear juice: a source of multiple nutraceutical and functional components. In: Scardina PG, editor Fruit juices. New York: Nova Science Publishers; 2009: 79-105
• 13 Chavez-Santoscoy RA, Gutierrez-Uribe JA, Serna-Saldivar SO. Phenolic composition, antioxidant capacity and in vitro cancer cell cytotoxicity of nine prickly pear (Opuntia spp.) juices. Plant Foods Hum Nutr 2009; 64: 146-152
• 14 Feugang JM, Ye F, Zhang DY, Yu Y, Zhong M, Zhang S, Zou C. Cactus pear extracts induce reactive oxygen species production and apoptosis in ovarian cancer cells. Nutr Cancer 2010; 62: 692-699
• 15 Pavokovic P, Krsnic-Rasol M. Complex biochemistry and biotechnological production of betanins. Food Technol Biotechnol 2011; 49: 145-155
• 16 Sreekanth D, Arunasree MK, Karnati R, Roy T, Chandramohan R, Reddy GV, Pallu R. Betanin, a betacyanin pigment purified from fruits of Opuntia ficus-indica induces apoptosis in human chronic myeloid leukemia cell line-K562. Phytomedicine 2007; 14: 739-746
• 17 Yoon JA, Hahm SW, Park J, Son YS. Total polyphenol and flavonoid of fruit extract of Opuntia humifusa and its inhibitory effect on the growth of MCF-7 human breast cancer cells. Hanʼguk Sikpʼum Yongyang Kwahak Hoechi 2009; 38: 1679-1684
• 18 Hahm SW, Park J, Son YS. Opuntia humifusa partitioned extracts inhibit the growth of U87MG human glioblastoma cells. Plant Foods Hum Nutr 2010; 65: 247-252
• 19 Chen-feng J, Xiang Z, Shi-yong G, Yu-bin J. Effects of three kinds of cactus polysaccharide on human cancer by MTT. Harbin Shangye Daxue Xuebao, Ziran Kexueban 2004; 20: 383-386
• 20 Brahmi D, Ayed Y, Bouaziz C, Zourgui L, Hassen W, Hassen BH. Hepatoprotective effect of cactus extract against carcinogenicity of benzo(a)pyrene on liver of Balb/C mice. J Med Plants Res 2011; 5: 4627-4639
• 21 Brahmi D, Bouaziz C, Ayed Y, Ben Mansour H, Zourgui L, Bacha H. Chemopreventive effect of cactus Opuntia ficus-indica on oxidative stress and genotoxicity of aflatoxin B1. Nutr Metab 2011; 8: 1-16
• 22 Brahmi D, Ayed Y, Hfaiedh M, Ben Mansour H, Bouaziz C, Zourgui L, Bacha H. Protective effect of cactus cladode extract against cisplatin induced oxidative stress, genotoxicity and apoptosis in balb/c mice: combination with phytochemical composition. BMC Complement Altern Med 2012; 12: 111
• 23 Lee JA, Jung BG, Lee BJ. Inhibitory effects of Opuntia humifusa on 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphobol-13-acetate induced two-stage skin carcinogenesis. Asian Pac J Cancer Prev 2012; 13: 4655-4660
• 24 Tan ML, Sulaiman SF, Najimuddin N, Samian MR, Tengku MTS. Methanolic extract of Pereskia bleo induces apoptosis in breast carcinoma (T-47D) cell line. J Ethnopharmacol 2005; 96: 287-294
• 25 Sidik NJ, Norihan MS, Shafii K. In vitro culture of Pereskia bleo . Acta Horticult 2009; 829: 99-104
• 26 Er HM, Cheng E, Radhakrishnan AK. Anti-proliferative and mutagenic activities of aqueous and methanol extracts of leaves from Pereskia bleo (Kunth) DC leaves. J Ethnopharmacol 2007; 113: 448-456
• 27 Sri Nurestri AM, Northanom AW, Hashim Y, Shi S, Sok LH, Lee GS, Syarifah NSAR. Cytotoxic activity of Pereskia bleo (Cactaceae) against selected human cell lines. Int J Cancer Res 2008; 4: 20-27
• 28 Malek SN, Shin SK, Wahab NA, Yaacob H. Cytotoxic components of Pereskia bleo (Kunth) DC. (Cactaceae) leaves. Molecules 2009; 14: 1713-1724
• 29 Sim KS, Sri Nurestri AM, Norhanom AW. Phenolic content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts. Pharmacogn Mag 2010; 6: 248-254
• 30 Sri Nurestri AM, Sim KS, Northanom AW. Phytochemical and cytotoxic investigations of Pereskia grandifolia Haw. (Cactaceae) leaves. J Biol Sci 2009; 9: 488-493
• 31 Liew SY, Stanbridge EJ, Yusoff K, Shafee N. Hypoxia affects cellular responses to plant extracts. J Ethnopharmacol 2012; 144: 453-456
• 32 Kim H, Choi HK, Moon JY, Kim YS, Mosaddik A, Cho SK. Comparative antioxidant and antiproliferative activities of red and white pitayas and their correlation with flavonoid and polyphenol content. J Food Sci 2011; 76: C38-C45
• 33 Wu LC, Hsu HW, Chen YC, Chiu CC, Lin YI, Ho JA. Antioxidant and antiproliferative activities of red pitaya. Food Chem 2006; 95: 319-327
• 34 Jayakumar R, Kanthimathi MS. Inhibitory effects of fruit extracts on nitric oxide-induced proliferation in MCF-7 cells. Food Chem 2011; 126: 956-960
• 35 Kim SM, Choi HJ, Kim YS. Anticancer composition containing extract of Hylocereus undatus as active ingredient. Repub Korean Kongkae Taeho Kongbo KR Patent 2012; 008370 A 2012013; 2012
• 36 Salazar JR, Martinez-Vazquez M, Cespedes CL, Ramirez-Apan T, Nieto-Camacho A, Rodriguez-Silverio J, Flores-Murrieta F. Anti-inflammatory and cytotoxic activities of chichipegenin, peniocerol, and macdougallin isolated from Myrtillocactus geometrizans . J Biosci 2011; 66: 24-30
• 37 Yetman D. The organ pipe cactus. Tucson: The University of Arizona Press; 2006
• 38 Kinoshita K, Yang Y, Koyama K, Takahashi K, Nishino H. Inhibitory effect of some triterpenes from cacti on Pi-incorporation into phospholipids of HeLa cells promoted by 12-O-tetradecanoylphorbol-13-acetate. Phytomedicine 1999; 6: 73-77
• 39 Nugent J. Permaculture plants agaves and cacti. Nannup: The Sustainable Agriculture Research Institute; 1999
• 40 Karimi N, Kianamiri S, Mousavi E, Barkhordar M. Study of genotype effect, different media and time of explanting on callus induction in Cereus peruvianus Mill. (Cactaceae). Trakia J Sci 2012; 10: 19-22
• 41 Guo LP. Review on extraction, structure and bioactivity of polysaccharides from cactus cladode. Zhongguo Shipin Tianjiaji 2010; 3: 185-189
• 42 Li YJ, Lai YH. Cactus biological functions and its pharmacological effects. Beihua Daxue Xuebao Ziran Kexueban 2007; 8: 228-231
• 43 Beltran JFR. Process for extracting and manufacturing cactus juice. Mex Pat Application MX 2008007250 A 20091207; 2009.
• 44 Wu D, Wei B, Wang S, Sun HX, Xin Y, Zhang CL. The inhibiting effect of wild cactus polysaccharide on lung squamous carcinoma cells (SK-MES-1). Xiandai Shengwuyixue Jinzhan 2012; 12: 1651-1654
• 45 Liang B, Liu H, Cao J. Antitumor effect of polysaccharides from cactus pear fruit in S180-bearing mice. Ai Zhang 2008; 27: 580-584
• 46 Ji CF, Zou X, Gao SY, Ji YB. Study on antitumor effect of three kinds of cactus polysaccharide. Harbin Shangye Daxue Xuebao Ziran Kexueban 2004; 20: 127-130
• 47 Ji YB, Ji CF, Zou X, Gao SY. Study on the effects of two kinds of cactus polysaccharide on erythrocyte immune function of S180 mice. China J Chin Mater Med 2005; 30: 690-693
• 48 Hu QL, Feng ZU. Effects of two cactus polysaccharides on membrane lipid fluidity of S180 tumor-bearing mice. Zhongguo Xiandai Zhongyao 2006; 8: 17-19