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Planta Med 2013; 79(11): 933-938
DOI: 10.1055/s-0032-1328649
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Dodoviscin A Inhibits Melanogenesis in Mouse B16-F10 Melanoma Cells

Guirui Yan
1   Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
,
Jingjie Zhu
1   Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
,
Laibin Zhang
2   Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, China
,
Zhijian Xu
1   Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
,
Gaihong Wang
1   Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
,
Weiliang Zhu
1   Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
,
Aijun Hou
2   Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, China
,
Heyao Wang
1   Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
› Author Affiliations
Further Information

Publication History

received 25 November 2012
revised 04 May 2013

accepted 12 May 2013

Publication Date:
26 June 2013 (online)

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Abstract

Nowadays, abnormal hyperpigmentation in human skin such as melasma, freckles, and chloasma has become a serious esthetic problem. Cutaneous depigmenting agents could be used to treat these hyperpigmentation-associated dieseases. Dodoviscin A is a natural product isolated from the aerial parts of Dodonaea viscosa. In the present study, we evaluated the effect of dodoviscin A on melanin production in B16-F10 melanoma cells for the first time. We found that dodoviscin A inhibited melanin biosynthesis induced by 3-isobutyl-1-methylxanthine and PD98059 significantly, and there was no obvious effect on the viability of dodoviscin A-treated B16-F10 cells. Meanwhile, dodoviscin A could suppress the activity of mushroom tyrosinase in the cell-free assay system and also decrease 3-isobutyl-1-methylxanthine-induced tyrosinase activity and expression of mature tyrosinase protein in B16-F10 cells. Western blotting analysis showed that dodoviscin A inhibited 3-isobutyl-1-methylxanthine and forskolin-induced phosphorylation of the cAMP response element binding protein in B16-F10 cells. These results indicate that dodoviscin A may be a new promising pigmentation-altering agent for cosmetic and therapeutic applications.

Key words

dodoviscin A - Dodonaea viscosa - Sapindaceae - melanogenesis - tyrosinase - CREB

Supporting Information

 

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