Abstract
Aim An experimental study was performed to evaluate the effect of whole gut irrigation
(WGI) solutions on contractile responses of the gallbladder and ileum and also on
tissue cholecystokinin (CCK) levels.
Materials and Methods Thirty guinea pigs were enrolled into five groups including control group (CG) and
WGI group (saline physiologic [SP], Ringer lactated [RL], polyethylene glycol [PEG],
and dibasic sodium phosphate [DNP]). After median laparotomy, the distal esophagus
was ligated and SP, PEG, RL, and DNP infusions (2 mL/kg/min) were performed via gastric
catheter until rectal discharge became clear in WGI groups. Ileum and gallbladder
samples were obtained for in vitro and biochemical studies without irrigation in CG
and after irrigation with different WGI solutions. Isolated ileum and gallbladder
preparations were suspended in organ baths for contractile responses of carbachol
and CCK. Also, biochemical analysis of tissue CCK levels was performed in ileum and
gallbladder samples.
Results In PEG group, gallbladder and ileum CCK levels were significantly higher than CG
(p < 0.05). Also, DNP irrigation caused increased CCK levels in gallbladder samples
(p < 0.05). In lower carbachol concentrations, PEG group showed increased contraction
responses in gallbladder samples when compared with controls (p < 0.05). However, ileal responses to carbachol did not show any significant difference
between groups, contraction responses to CCK was decreased in PEG group when compared
with CG (p < 0.05).
Conclusion Among WGI solutions, PEG caused the highest CCK levels in gallbladder and ileum samples.
Different WGI solutions affected the contractile responses of gallbladder and ileum
smooth muscles divergently. Increased levels of CCK in PEG group support the decreased
contractile responses in ileum. Therefore, our results confirm that the effect of
WGI on gallbladder and ileum contractility may be CCK related.
Keywords
whole gut irrigation - gallbladder - cholecystokinin
