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Planta Med 2013; 79(17): 1636-1640
DOI: 10.1055/s-0033-1350954
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Cyanidin-3-O-Sambubioside from Acanthopanax sessiliflorus Fruit Inhibits Metastasis by Downregulating MMP-9 in Breast Cancer Cells MDA-MB-231

Sang-Jin Lee
1   Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Womenʼs University, Seoul, Republic of Korea
2   Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea
,
Sungyoul Hong
2   Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea
,
Sang-Ho Yoo
3   Department of Food Science & Technology and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, Republic of Korea
,
Gye-Won Kim
4   Brewing Research Center, Academic Industry Cooperation, Hankyong National University, Ansung, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 25 February 2013
revised 11 September 2013

accepted 23 September 2013

Publication Date:
08 November 2013 (online)

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Abstract

Anthocyanins play an important role in physiological functions related to human health. This study aimed to investigate the inhibitory effect of cyanidin-3-O-sambubioside isolated from Acanthopanax sessiliflorus fruit on cancer cell metastasis. The inhibitory effect of cyanidin-3-O-sambubioside on angiogenesis was assessed by chorioallantoic membrane and tube formation assays. Cyanidin-3-O-sambubioside interrupted the formation of neovasculature and tube-like structures in a dose-dependent manner. It inhibited the wound healing migration and invasion of MDA-MB-231 cells through reconstituted extracellular matrix (Matrigel). Gelatin zymography revealed that cyanidin-3-O-sambubioside significantly decreased the secretion of matrix metalloproteinase-9 and inhibited matrix metalloproteinase-9 expression. In additional, cyanidin-3-O-sambubioside exerted an inhibitory effect on Akt phosphorylation. These results suggest that cyanidin-3-O-sambubioside inhibits metastasis processes, such as angiogenesis and invasion, in breast cancer cells through regulation of matrix metalloproteinase-9 activity.

Key words

angiogenesis - cancer metastasis - cyanidin-3-O-sambubioside - fruit of Acanthopanax sessiliflorus - Araliaceae - invasion - MMP-9
 

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