Antiallergic Effects of Pigments Isolated from Green Sea Urchin
                        (Strongylocentrotus droebachiensis) Shells

Olga N. Pozharitskaya; Alexander N. Shikov; Marina N. Makarova; Svetlana A. Ivanova; Vera M. Kosman; Valery G. Makarov; Václav Bazgier; Karel Berka; Michal Otyepka; Jitka Ulrichová

doi:10.1055/s-0033-1351098

Planta Medica, Inhaltsverzeichnis

Planta Med 2013; 79(18): 1698-1704
DOI: 10.1055/s-0033-1351098

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Antiallergic Effects of Pigments Isolated from Green Sea Urchin (Strongylocentrotus droebachiensis) Shells

Autor*innen

Olga N. Pozharitskaya

¹Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
Alexander N. Shikov

¹Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
Marina N. Makarova

¹Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
Svetlana A. Ivanova

¹Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
Vera M. Kosman

¹Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
Valery G. Makarov

¹Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia
Václav Bazgier

²Department of Physical Chemistry, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
Karel Berka

³Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic
Michal Otyepka

³Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic
Jitka Ulrichová

⁴Department of Medical Chemistry and Biochemistry, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic

Abstract

This study was undertaken to evaluate possible antiallergic effects of an extract of pigments from green sea urchin (Strongylocentrotus droebachiensis) shells. Effects were studied on animal models – guinea pig ileum contraction, rabbit eyes allergic conjunctivitis, and rabbit local skin irritation. The extract significantly reduced, in a dose-dependent manner, the histamine-induced contractions of the isolated guinea pig ileum with ID₅₀ = 1.2 µg/mL (in equivalents of spinochrome B), had an inhibitory effect on the model of ocular allergic inflammation surpassing the reference drug olopatadine, and did not show any irritating effect in rabbits. The extract predominantly contained polyhydroxy-1,4-naphthoquinone which would be responsible for the pharmacological activity. The active compounds of the extract were evaluated in silico with molecular docking. Molecular docking into H₁R receptor structures obtained from molecular dynamic simulations showed that all spinochrome derivatives bind to the receptor active site, but spinochrome monomers fit better to it. The results of the present study suggest possibilities for the development of new agents for treating allergic diseases on the base of pigments from sea urchins shells.

Key words

Strongylocentrotus droebachiensis - Strongylocentrotidae - spinochrome - histamine H₁-receptor - guinea pig ileum - allergic conjunctivitis - molecular docking

Volltext

Referenzen

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