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Eur J Pediatr Surg 2015; 25(03): 299-304
DOI: 10.1055/s-0034-1371715
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Beneficial Effects of Methylene Blue on the Acute Phase of Corrosive Esophageal Burn in Rats

Huseyin Kilincaslan
1   Department of Pediatric Surgery, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
,
Hande Ozgun Karatepe
2   Department of Pediatrics, Zeynep Kamil Research and Education Hospital, Istanbul, Turkey
,
Elif Kilic
3   Department of Biochemistry, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
,
Ibrahim Aydogdu
1   Department of Pediatric Surgery, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
,
Ahmet Hakan Gedik
4   Department of Pediatrics, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
,
Vakur Olgac
5   Department of Pathology, Institute of Oncology, Istanbul University, Istanbul, Turkey
,
Mehmet Kucukkoc
4   Department of Pediatrics, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
› Author Affiliations
Further Information

Publication History

26 August 2013

25 January 2014

Publication Date:
04 April 2014 (online)

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Abstract

Introduction The aim of the article is to investigate the beneficial effects of methylene blue (MB) on the acute phase of experimental corrosive esophageal burns.

Materials and Methods A total of 21 male Sprague-Dawley rats were allocated into three groups with an equal number in each group. In Group 1 (control), the esophageal lumen was irrigated with saline. In Groups 2 (untreated) and 3 (MB treatment), the distal esophagus was burned with 30% NaOH solution. In Group 3, MB was applied locally on the distal esophageal lumen (30 mg/kg) 90 minutes after the burn. In all the groups, animals were humanely killed at the end of the 7th day. Distal esophageal specimens were obtained for histopathological and biochemical examinations.

Results MB increased the tissue superoxide dismutase levels and the total antioxidant status, whereas it decreased the levels of the total oxidant status with significant attenuation of histopathological damage.

Conclusion MB might have reduced the tissue damage in corrosive esophagitis because of its antioxidant effects.

Keywords

corrosive esophageal burn - methylene blue - total oxidant status - total antioxidant status
 

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