A Chicken Embryo Model for the Study of Umbilical and Supraumbilical Body Wall Malformations

Ina Ridderbusch; Robert Bergholz; Miriam Fattouh; Georg Eschenburg; Beate Roth; Birgit Appl; Joerg Maenner; Konrad Reinshagen; Dietrich Kluth

doi:10.1055/s-0034-1371718

European Journal of Pediatric Surgery, Table of Contents

Eur J Pediatr Surg 2015; 25(03): 257-261
DOI: 10.1055/s-0034-1371718

Original Article

Georg Thieme Verlag KG Stuttgart · New York

A Chicken Embryo Model for the Study of Umbilical and Supraumbilical Body Wall Malformations

Authors

Ina Ridderbusch

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

^*Both authors contributed equally to the manuscript.
Robert Bergholz

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

^*Both authors contributed equally to the manuscript.
Miriam Fattouh

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Georg Eschenburg

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Beate Roth

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Birgit Appl

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Joerg Maenner

²Department of Anatomy and Embryology, University of Goettingen, Goettingen, Lower Saxony, Germany
Konrad Reinshagen

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Dietrich Kluth

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

Abstract

Background/Purpose The embryology of ventral body wall malformations is only partially understood, although their incidence is relatively common. As only few experimental data exist on the development of those defects, the aim of our study was to compare the teratogenic effect of trypan blue (TB) and suramin (SA) in their capability to induce umbilical and supraumbilical abdominal wall malformations in a chicken egg model.

Materials and Methods A total of 255 fertilized chicken eggs were incubated at 38°C and 75% relative humidity. Embryos were treated in ovo on incubation day 2.5 (Hamburger/Hamilton (HH) stage 13). The eggshell was windowed, and solutions of TB or SA were injected into the coelomic cavity at the region of the umbilicus. The window was closed and the embryos reincubated until examination on day 8 (HH 34).

Results A total of 60 embryos survived in each group. The largest number of embryos presented with defects in the umbilical and supraumbilical region (25% in the SA group and 40% in the TB group). A combination of both defects (thoracoabdominoschisis) was seen in 20% of the TB and 8.3% of the SA groups, respectively. Associated anomalies found in both groups were head and eye defects, abnormal pelvic configurations, leg deformities, and mild forms of cloacal exstrophies.

Conclusions TB and SA have both a high potential to induce umbilical and supraumbilical ventral body wall malformations in chicken embryos. This novel animal model might help to establish a more profound understanding of the developmental steps in ventral body wall formation and the embryology for its malformations.

Keywords

omphalocele - chicken embryo model - abdominal wall defects

Full Text

References

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