Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596215
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Anti-biofilm potential of some plant volatile compounds against Proteus mirabilis

Autoren

  • JAA do Nascimento Júnior

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • BS dos Santos

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • LCA de Araújo

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • AVA Lima

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • TD da Silva

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • LCN da Silva

    2   Post-Graduate Program in Parasite Biology, University of CEUMA, Rua Josué Montello 1 65.075 – 120, São Luís, Brazil

  • TH Napoleão

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • PMG Paiva

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • MV da Silva

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil

  • MT dos Santos Correia

    1   Department of Biochemistry, Federal University of Pernambuco, Avenida Professor Moraes Rêgo 50670 – 420, Recife, Brazil
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
14. Dezember 2016 (online)

Auch verfügbar auf
 
 

Microbial biofilms are structured communities of cells enclosed in a polymeric matrix which allows the adherence to inert or living surfaces. This structure ensure the growth even in hostile environments, for example plantonic cells are usaully more senstive to antimicrobials than biofilms [1,2]. Proteus mirabilis (Gram-negative) is a uropathogenic bacteria well known for its ability to form biofilm and related to catheter-associated urinary tract infections. In this context, there is a great need to search new therapies for control or prevent infections targeting biofilm formation. Plant derived produtcs still provide a potential source of new drugs due they structure diversity. Among them, the essential oils (complex mixtures of volatile compounds) have been highlighted due their antimicrobial and antibiofilm action [3,4,5]. The aim of this work is to investigate the antibiofilm activity of some volatile compounds (caryophyllene oxide, β-caryophyllene, α-pinene, octanoic acid, dodecanoic acid, and decanoic acid) widely reported as major compounds of essential oils against the uropathogenic bacteria Proteus mirabilis. Initially subinhibitory concentrations were determined. The ability to inhibit or detachment was performed using biofilm quantitification assay [6]. The growth of P. mirabilis was not affected by any compounds at the highest tested concentration (2 mg/mL). On the hand, all compounds prevented the biofilm formation with inhibitory values ranged from 42% to 82% at 2 mg/mL and 32 to 75% at 1 mg/mL. The best inhibitor was decanoic acid (p < 0.05). In addition, all compounds induced a significative detachment of biofilm formed for 24h (except dodecanoic acid). The maximum detachment was observed with octanoic acid (99%; p < 0.05). In conclusion, our results revealed new agents with anti-biofilm properties, specially octanoic and decanoic acids. These compounds may be used as lead compound to direct programs for biomedical applications.

Acknowledgements: Brazilian agencies: CAPES, FACEPE, CNPq.

Keywords: Antivirulence, biofilm inhibition, essential oils.

References:

[1] Khan MSA, Lee J. Novel Strategies for combating pathogenic biofilms using plant products and microbial antibiosis. Current Pharmaceutical Biotechnology 2016, 17, 126 – 140

[2] Richards J, Melander C. Controlling bacterial biofilms. Chembiochem 2009; 10: 2287 – 2294

[3] Sutherland IW. Biofilm exopolysaccharides: a strong and sticky framework. Microbiol 2001; 147: 3 – 9

[4] Cogan NG, Cortez R, Fauci L. Modeling physiological resistance in bacterial biofilms. Bulletin of Mathematical Biology 2005; 67: 831 – 853

[5] Burt, S. Essential oils: their antibacterial properties and potential applications in foods – a review. IntJ Food Microbiol 2004; 94: 223 – 253

[6] Trentin DS, Giordani RB, Zimmer KR, Silva AG, Silva MV, Correia MT, Baumvold, IJR, Macedo AJ. Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles. J Ethnopharmacol 2011; 137: 327 – 335


Die Autoren geben an, dass kein Interessenkonflikt besteht.