Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596752
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Assessment of the bactericidal effect of green synthesized silver nanoparticles against a panel of infectious microorganisms

Authors

  • E Antunes

    1   Department of Chemistry, University of the Western Cape, Robert Sobukwe Rd, Bellville, 7535, Cape Town, South Africa

  • M Mmola

    2   Department of Biotechnology, University of the Western Cape, Robert Sobukwe Rd, Bellville, 7535, Cape Town, South Africa
    3   School of Pharmacy, University of the Western Cape, Robert Sobukwe Rd, Bellville, 7535, Cape Town, South Africa

  • M Meyer

    2   Department of Biotechnology, University of the Western Cape, Robert Sobukwe Rd, Bellville, 7535, Cape Town, South Africa

  • DR Beukes

    3   School of Pharmacy, University of the Western Cape, Robert Sobukwe Rd, Bellville, 7535, Cape Town, South Africa
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Publikationsverlauf

Publikationsdatum:
14. Dezember 2016 (online)

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    The emergence of multi-drug resistant microorganisms poses a major threat to human life, making the antibiotics currently in use, ineffective [1]. There is therefore a need for the development of new broad-spectrum antibiotics. Current research has been channelled into nanoscience in conjunction with drug discovery, in the search for effective antibacterial agents. Nanoscience is the study of the remarkable properties a material exhibits when it is reduced to less than 100nm in size. The synthesis of nanoparticles can be achieved using the so called “bottom-up” or “top-down” approaches. However, the chemicals employed are typically toxic or harmful, restricting their use in medical applications. There is therefore a need for the use of eco-friendly, nanoparticle synthetic methods i.e. “green chemistry” methods [2,3].

    The synthesis of the silver and gold nanoparticles (NPs) used in this study were carried out by means of green synthetic methods, using an aqueous extract from a South African endemic brown alga Sargassum incisifolium. For comparison, commercially available brown algal fucoidans were also used to synthesise the NPs. The NPs were characterised using UV-Vis and FT-IR spectroscopy, Transmission electron microscopy, Dynamic light scattering, zeta potential measurements and X-ray diffraction. The rate of NP formation clearly varied with the type of reducing agent used and the NPs produced varied in size from 5nm to as much as 66nm for both sets of NPs. The AgNPs synthesised using the Sargassum aqueous extracts showed excellent antimicrobial activity against five pathogenic microorganisms including A. baumannii, K. pneumoniae, E. faecalis, S. aureus, and C. albicans, while the AuNPs were found to be much less effective. The cytotoxic activity of these NPs was also assessed against three cell lines, namely MCF-7, HT-29 and MCF-12a. The AgNPs were found to be toxic to both the HT-29 and MCF-7 cell lines, exhibiting slightly less toxicity to the MCF-12a cells. The AuNPs showed lower toxicity levels.

    Acknowledgements: The authors gratefully acknowledge UWC, NRF CSUR and CPRR grants (South Africa) and the DST/National Nanoscience Postgraduate Teaching and Training Programme (NNPTTP) for financial support.

    Keywords: Green synthesis, fucoidans, Sargassum incisifolium, antibacterial activity, cytotoxicity.

    References:

    [1] Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ. The bactericidal effect of silver nanoparticles. Nanotechnol 2005; 16: 2346 – 2353

    [2] Sun Q, Cai X, Li J, Zheng M, Chen Z, Yu CP. Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids Surf A 2014; 444: 226 – 231

    [3] Akhtar MS, Panwar J, Yun Y. Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustainable Chem Eng 2013; 1: 591 – 602


     

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