Synfacts 2019; 15(04): 0439
DOI: 10.1055/s-0037-1612404
Chemistry in Medicine and Biology
© Georg Thieme Verlag Stuttgart · New York

Synthesis of Human Interleukin-8 by Native Chemical Ligation

Dirk Trauner
Julius R. Reyes
Dawson PE, Muir TW, Clark-Lewis I, Kent SB. * The Scripps Research Institute, La Jolla, USA
Synthesis of Proteins by Native Chemical Ligation.

Science 1994;
266: 776-779
Further Information

Publication History

Publication Date:
19 March 2019 (online)



The realization that unprotected peptide thioesters react chemoselectively with unprotected N-terminal cysteine residues to form amide linkages is an important advancement in protein synthesis. Native Chemical Ligation (NCL) allows synthetic access to proteins of moderate size.



NCL proceeds by the formation of an intermediate thioester linkage with cysteine that then spontaneously rearranges to the native amide bond. This strategy was applied to the total chemical synthesis of human interleukin 8 (IL-8), a 72-amino acid protein containing four cysteine residues.