Polyphenolics from Romanian native medicinal plants and used in veterinary medicine

Flavonoids and polyphenols are widely distributed in medicinal plants and have positive health effects. The aim of this study were to determine the flavonoids and polyphenols with a spectrophotometrical and by an UHPLC-LC-MS/MS method [1].

Eighteen widely used medicinal plants were studied. Total flavonoids and total polyphenolics content were determined quantitatively by spectrophotometrical method from the methanolic and ethanolic (70⁰) extracts of plants. Additionally an UHPLC-LC-MS/MS method was developed for determination of seven standards from extracts. Detection was carried out in SRM mode (chlorogenic acid, caffeic acid, quercitrin, luteolin, quercetin, apigenin, kaempferol)l.

Medicinal plants are a rich source of flavonoids and polyphenolics. The seven standard were detectabled in high concentration from Hyperici herba, Betulae folium and Potentillae herba [Fig.1.].

Fig. 1

There are a wide variety of phenolics and flavonoids and the obtained antioxidant values confirms a high antioxidant capacities of extracts. For the determination of polyphenolic profile the developed UHPLC-DAD-QTOF-MS/MS method is fast and acceptable. Polyphenols may be developed in veterinary products and used for animals.

 

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Acknowledgements

This work was supported by the University of Medicine, Pharmacy Sciences and Technology of Târgu Mureș and SC Promedivet SRL, under internal research grant number 17972/07.12.2016.

• References

• 1 Parets L, Alechaga E, Nunez O, Saurina J, Hernandez-Cassou S, Puignou L. Ultrahigh pressure liquid chromatography-atmospheric pressure photoionization-tandem mass spectromerty for the determination of polyphenolic profiles in the characterization and classification of cranberry-based pharmaceutical preparations and natural extracts. Anal Methods_UK 2016; 8 (22) 4363-4378.