Amentoflavone Inhibits Osteoclastogenesis and Wear Debris-Induced Osteolysis via Suppressing NF-κB and MAPKs Signaling Pathways

Zhen Zhang; Shuai Zhao; Xiaolei Li; Xiaoqi Zhuo; Wu Zhang; Qian Nie; Shuguang Wang; Lianqi Yan; Yu Sun

doi:10.1055/s-0043-124594

Planta Medica, Table of Contents

Planta Med 2018; 84(11): 759-767
DOI: 10.1055/s-0043-124594

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Amentoflavone Inhibits Osteoclastogenesis and Wear Debris-Induced Osteolysis via Suppressing NF-κB and MAPKs Signaling Pathways

Authors

Zhen Zhang

¹Dalian Medical University, Dalian, Liaoning, China

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China
Shuai Zhao

⁴Xiangya Second Hospital, Central South University, Changsha, Hunan, China
Xiaolei Li

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China

⁴Xiangya Second Hospital, Central South University, Changsha, Hunan, China
Xiaoqi Zhuo

³Peking University Third Hospital, Peking University, Beijing, China
Wu Zhang

¹Dalian Medical University, Dalian, Liaoning, China

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China
Qian Nie

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China
Shuguang Wang

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China
Lianqi Yan

¹Dalian Medical University, Dalian, Liaoning, China

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China

⁴Xiangya Second Hospital, Central South University, Changsha, Hunan, China
Yu Sun

²Department of Orthopedics, Subei Peopleʼs Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, China

Abstract

Wear debris-induced osteolysis is one of the major reasons for subsequent aseptic loosening after cementless hip arthroplasty. Increasing evidence suggests that receptor activator of nuclear factor kappa-B (NF-κB) ligand-mediated osteoclastogenesis and osteolysis are responsible for wear debris-induced aseptic loosening. In the present study, we explored the effect of amentoflavone (AMF) on inhibiting osteoclast generation and wear debris-induced osteolysis in vitro and in vivo. Twenty-four male C57BL/J6 mice were randomly divided into four groups: a sham group and groups with titanium wear debris treatment followed by intraperitoneal injection of various concentrations of AMF (0, 20, and 40 mg/kg/day). The micro computed tomography scanning and histological analysis were performed. Bone marrow-derived macrophages were cultured to investigate the effect of AMF on osteoclast generation and function. The results showed that AMF suppressed osteoclastogenesis, F-actin ring formation, and bone absorption without cytotoxicity. AMF prevented titanium wear debris-induced osteolysis in mice. AMF suppressed the relative proteins of NF-κB and mitogen-activated protein kinase (MAPKs) signaling pathways. Thus, the present study suggests that AMF derived from plants could inhibit osteoclastogenesis and titanium wear debris-induced osteolysis via suppressing NF-κB and MAPKs signaling pathways.

Key words

amentoflavone - osteoclastogenesis - osteolysis - RANKL - MAPKs - NF-κB

Full Text

References

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