Download PDF
J Pediatr Infect Dis 2023; 18(05): 250-255
DOI: 10.1055/s-0043-1770989
Original Article

Antibiofilm Activity of Different Washing Solutions against Biofilms in Cystic Fibrosis Patients' Nasal Cavities

Gökhan Tüzemen 
1   Department of Otorhinolaryngology-Head and Neck Surgery, Bursa City Hospital, Mınıstry of Health, Bursa, Türkiye
,
Nazmiye Ülkü Tüzemen 
2   Department of Medical Microbiology, Faculty of Medicine, Bursa Uludag University, Bursa, Türkiye
,
Perihan Erkan Alkan 
3   Department of Medical Microbiology, Faculty of Medicine, Bandırma Onyedi Eylül University, Bandırma, Balıkesir, Türkiye
,
Cüneyt Özakın 
2   Department of Medical Microbiology, Faculty of Medicine, Bursa Uludag University, Bursa, Türkiye
› Author Affiliations
› Further Information
    Permissions and Reprints

Abstract

Objective Bacterial biofilm formation is a multistep process involving bacterial adhesion to inorganic or mucosal surfaces. We aimed to identify Staphylococcus aureus and Pseudomonas aeruginosa strains colonizing the respiratory tracts of cystic fibrosis (CF) patients and to gauge the antibiofilm potential of streptomycin and ozone solutions against them.

Methods Bacteria were obtained from CF patients' sputum samples processed in our microbiology laboratory over 1 year (2021–2022). A total of 26 nonduplicate strains (13 S. aureus and 13 P. aeruginosa) were included in this study.

Results Both ozone and streptomycin solutions showed significant inhibitory activity. However, when faced with mature biofilm, the streptomycin solution had a significantly more substantial impact than the ozone solution. Furthermore, the ozone solution had no inhibitory effect on mature P. aeruginosa biofilm.

Conclusion Ozone and streptomycin solutions might be used as nasal irrigation to eliminate the biofilms in patients with CF in acute respiratory infections. However, our in vitro observations would need to be confirmed in vivo. In chronic inflammation, ozone solution cannot degrade the mature biofilm of P. aeruginosa, whereas streptomycin solution can degrade such biofilms. This result is promising in lessening the biofilms associated with these bacteria that colonize patients with CF.

Keywords

antibiofilm - S. aureus - P. aeruginosa - ozone - streptomycin


Publication History

Received: 09 February 2023

Accepted: 31 May 2023

Article published online:
24 July 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Johnson BJ, Choby GW, O'Brien EK. Chronic rhinosinusitis in patients with cystic fibrosis-current management and new treatments. Laryngoscope Investig Otolaryngol 2020; 5 (03) 368-374
    CrossrefPubMedGoogle Scholar
  • 2 Kim YS, Kim NH, Seong SY, Kim KR, Lee GB, Kim KS. Prevalence and risk factors of chronic rhinosinusitis in Korea. Am J Rhinol Allergy 2011; 25 (03) 117-121
    CrossrefPubMedGoogle Scholar
  • 3 Xu X, Reitsma S, Wang Y, Fokkens WJ. Highlights in the advances of chronic rhinosinusitis. Allergy 2021; 76 (11) 3349-3358
    CrossrefPubMedGoogle Scholar
  • 4 Tóth L, Csomor P, Sziklai I, Karosi T. Biofilm detection in chronic rhinosinusitis by combined application of hematoxylin-eosin and gram staining. Eur Arch Otorhinolaryngol 2011; 268 (10) 1455-1462
    CrossrefPubMedGoogle Scholar
  • 5 Lee S, Lane AP. Chronic rhinosinusitis as a multifactorial inflammatory disorder. Curr Infect Dis Rep 2011; 13 (02) 159-168
    CrossrefPubMedGoogle Scholar
  • 6 Rather MA, Gupta K, Mandal M. Microbial biofilm: formation, architecture, antibiotic resistance, and control strategies. Braz J Microbiol 2021; 52 (04) 1701-1718
    CrossrefPubMedGoogle Scholar
  • 7 Fineberg NL. Streptomycin used locally in the care of chronic suppurative maxillary sinusitis; report of a case. Laryngoscope 1948; 58 (06) 545-551
    CrossrefPubMedGoogle Scholar
  • 8 Khan F, Lee JW, Pham DTN. et al. Streptomycin mediated biofilm inhibition and suppression of virulence properties in Pseudomonas aeruginosa PAO1. Appl Microbiol Biotechnol 2020; 104 (02) 799-816
    CrossrefPubMedGoogle Scholar
  • 9 Mansouri M, Khayam N, Jamshidifar E. et al. Streptomycin sulfate-loaded niosomes enables increased antimicrobial and anti-biofilm activities. Front Bioeng Biotechnol 2021; 9: 745099
    CrossrefPubMedGoogle Scholar
  • 10 Silva V, Peirone C, Amaral JS. et al. High efficacy of ozonated oils on the removal of biofilms produced by methicillin-resistant Staphylococcus aureus (MRSA) from infected diabetic foot ulcers. Molecules 2020; 25 (16) 3601
    CrossrefPubMedGoogle Scholar
  • 11 Sabancı AU, Erkan Alkan P, Mujde C. et al. Nanobubble ozone stored in hyaluronic acid decorated liposomes: antibacterial, anti-SARS-CoV-2 effect and biocompatibility tests. Int J Nanomedicine 2022; 17: 351-379
    CrossrefPubMedGoogle Scholar
  • 12 Stepanovic S, Vukovic D, Dakic I, Savic B, Svabic-Vlahovic M. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 2000; 40 (02) 175-179
    CrossrefPubMedGoogle Scholar
  • 13 Di Paolo N, Bocci V, Gaggiotti E. Ozone therapy. Int J Artif Organs 2004; 27 (03) 168-175
    CrossrefPubMedGoogle Scholar
  • 14 Chusri S, Phatthalung PN, Voravuthikunchai SP. Anti-biofilm activity of Quercus infectoria G. Olivier against methicillin-resistant Staphylococcus aureus . Lett Appl Microbiol 2012; 54 (06) 511-517
    CrossrefPubMedGoogle Scholar
  • 15 Song YJ, Yu HH, Kim YJ, Lee NK, Paik HD. Anti-biofilm activity of grapefruit seed extract against Staphylococcus aureus and Escherichia coli. . J Microbiol Biotechnol 2019; 29 (08) 1177-1183
    CrossrefPubMedGoogle Scholar
  • 16 Ghane M, Babaeekhou L, Ketabi SS. Antibiofilm activity of Kefir Probiotic Lactobacilli against uropathogenic Escherichia coli (UPEC). Avicenna J Med Biotechnol 2020; 12 (04) 221-229
    PubMedGoogle Scholar
  • 17 Rabin N, Zheng Y, Opoku-Temeng C, Du Y, Bonsu E, Sintim HO. Biofilm formation mechanisms and targets for developing antibiofilm agents. Future Med Chem 2015; 7 (04) 493-512 Erratum in: Future Med Chem 2015;7(10):1362
    CrossrefPubMedGoogle Scholar
  • 18 Nazzari E, Torretta S, Pignataro L, Marchisio P, Esposito S. Role of biofilm in children with recurrent upper respiratory tract infections. Eur J Clin Microbiol Infect Dis 2015; 34 (03) 421-429
    CrossrefPubMedGoogle Scholar
  • 19 Shaghayegh G, Cooksley C, Ramezanpour M, Wormald PJ, Psaltis AJ, Vreugde S. Chronic rhinosinusitis, S. aureus biofilm and secreted products, inflammatory responses, and disease severity. Biomedicines 2022; 10 (06) 1362
    CrossrefPubMedGoogle Scholar
  • 20 Tuon FF, Dantas LR, Suss PH, Tasca Ribeiro VS. Pathogenesis of the Pseudomonas aeruginosa biofilm: a review. Pathogens 2022; 11 (03) 300
    CrossrefPubMedGoogle Scholar
  • 21 Idrees M, Sawant S, Karodia N, Rahman A. Staphylococcus aureus biofilm: morphology, genetics, pathogenesis and treatment strategies. Int J Environ Res Public Health 2021; 18 (14) 7602
    CrossrefPubMedGoogle Scholar
  • 22 Kumar A, Ting YP. Streptomycin favors biofilm formation by altering cell surface properties. Appl Microbiol Biotechnol 2016; 100 (20) 8843-8853
    CrossrefPubMedGoogle Scholar
  • 23 Henry-Stanley MJ, Hess DJ, Wells CL. Aminoglycoside inhibition of Staphylococcus aureus biofilm formation is nutrient dependent. J Med Microbiol 2014; 63 (Pt 6): 861-869
    CrossrefPubMedGoogle Scholar
  • 24 Li R, Yuan X, Wei J. et al. Synthesis and evaluation of a chitosan oligosaccharide-streptomycin conjugate against Pseudomonas aeruginosa biofilms. Mar Drugs 2019; 17 (01) 43
    CrossrefPubMedGoogle Scholar
  • 25 Jiang Y, Xu Q, Jiang L, Zheng R. Isolation and characterization of a lytic Staphylococcus aureus phage WV against Staphylococcus aureus biofilm. Intervirology 2021; 64 (04) 169-177
    CrossrefPubMedGoogle Scholar
  • 26 Zerillo L, Polvere I, Varricchio R. et al. Antibiofilm and repair activity of ozonated oil in liposome. Microb Biotechnol 2022; 15 (05) 1422-1433
    CrossrefPubMedGoogle Scholar
  • 27 Panebianco F, Rubiola S, Chiesa F, Civera T, Di Ciccio PA. Effect of gaseous ozone treatment on biofilm of dairy-isolated Pseudomonas spp. strains. Ital J Food Saf 2022; 11 (02) 10350
    PubMedGoogle Scholar
  • 28 Piletić K, Kovač B, Perčić M. et al. Disinfecting action of gaseous ozone on OXA-48-producing Klebsiella pneumoniae biofilm in vitro. Int J Environ Res Public Health 2022; 19 (10) 6177
    CrossrefPubMedGoogle Scholar