Short Lecture “Angelica L. coumarins potential to modulate glucose- induced insulin secretion and their mechanism of action”

Pancreatic β-cell function and notably glucose-induced insulin secretion play a pivotal role in glucose homeostasis. We have previously shown that natural molecules like flavonoids or ellagitannin metabolites potentiate glucose-induced insulin secretion through a mechanism implicating the activation of L-type Ca²⁺(CaV) channels and ERK 1/2 [1] [2] [3]. According to some studies, extracts from Angelica L. species showed similar effects on insulin secretion [4] [5]. Thus, this study aimed at isolating and studying active constituents of three pharmacopeial Angelica roots.

Twenty-two coumarins were isolated from the roots of Angelica archangelica L., Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav., and Angelica pubescens Maxim through Soxhlet extraction in n-hexane, and column and preparative chromatography methods. The structures of the compounds were analysed and confirmed using UHPLC-DAD-MS and NMR methods. Pharmacological experiments were performed on the INS-1 β-cell line. Insulin release was quantified by the homogeneous time- resolved fluorescence method. The mechanism of action was studied using patch clamp and FACS techniques.

Some of the studied compounds were able to modulate glucose-induced insulin secretion by acting on the membrane potential, as well as by activating voltage-gated calcium channels and increasing intracellular calcium concentrations. Overall, the results demonstrate a new pharmacological activity of coumarins present in traditionally used Angelica roots.

Please note: article SL-YRW-07 was changed according to the following erratum: The authors name Soufi yan Omhmmed was misspelled, which is now corrected.

 

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• References

• 1 Bardy G, Virsolvy A, Quignard JF. et al. Quercetin induces insulin secretion by direct activation of L-type calcium channels in pancreatic beta cells. British Journal of Pharmacology 2013; 169: 1102-1113
• 2 Bayle M, Neasta J, Dall'Asta M. et al. The ellagitannin metabolite urolithin C is a glucose-dependent regulator of insulin secretion through activation of L-type calcium channels. Br J Pharmacol 2019; 176: 4065-4078
• 3 Toubal S, Oiry C, Bayle M. et al. Urolithin C increases glucose-induced ERK activation which contributes to insulin secretion. Fundam Clin Pharmacol 2020; 34: 571-580
• 4 Leu YL, Chen YW, Yang CY. et al. Extract isolated from Angelica hirsutiflora with insulin secretagogue activity. J Ethnopharmacol 2009; 123: 208-212
• 5 Park EY, Kim EH, Kim CY. et al. Angelica dahurica Extracts lmprove Glucose Tolerance through the Activation of GPR119. PLoS One 2016; 11: e0158796

Publication History

Article published online:
16 November 2023