Keynote Lecture 10 “Centrifugal Partition Chromatography (CPC) – principles and applications in the recovery of natural products of pharmacological significance”

Centrifugal partition chromatography (CPC) is a type of counter-current chromatography, and an advanced form of liquid chromatography (LC) that was developed in 1964 and since then used for the separation of compounds present in the mixtures (mainly of plant origin). The CPC technique offers several advantages in terms of operation, including no need for a solid sorbent (the separation is performed between two immiscible solvent mixtures, one of which plays a role of the stationary phase and the other – of the mobile phase), low operation temperatures, a wide range of separating solvents of reagent grade to be applied, or an easy up-scaling protocol, which enables its application in preparative or industrial volumes [1].

Apart from the introduction of this valuable technique, the aim of the lecture is to discuss about the possibilities that CPC may offer for plant drug delivery. Particular attention will be paid to the isolation of specialized metabolites with Central Nervous System targeting activity.

Details on the separation conditions, preparation of biphasic solvent systems and apparatus settings will be presented to discuss the efficiency of separation procedures in the recovery of plant derived secondary metabolites, e.g. from turmeric, ginger, horse chestnut, or others.

CPC is a powerful time- and reagent- saving separation technique providing high purity natural products even directly from crude extracts, and it covers analytical, preparative, and industrial scale operating conditions to efficiently facilitate the natural products recovery for medicinal purposes.

Funding The research was partially funded by the OPUS project from the National Science Centre in Poland (Grant number: 2021/41/B/NZ4/00337).

 

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Conflict of Interest

The authors declare no conflict of interest.

• References

• 1 Foucault AP, Chevolot L.. Counter-current chromatography: Instrumentation, solvent selection and some recent applications of natural product purification. J Chrom A 1998; 808: 3-22

Publication History

Article published online:
16 November 2023