Short Lecture "Development of analytical methods for the quantification ofanti-oomycete diterpenoids from larch extracts"

The viticulture industry is the fastest growing sector in UK agriculture and is producing award winning wines. Green Laryxine is an Innovate UK funded project aiming to commercialise the use of Larixyne​, a product containing two diterpenoids, larixyl acetate (1) and larixol (2) ([Fig. 1]), from European larch bark, which are active against grapevine downy mildew, Plasmopara viticola, that can cause 100% crop loss if left untreated. Currently, control of downy mildew in vineyards relies on copper fungicides that have unfavourable ecotoxicological profiles. Larixyne is effective in controlling downy mildew in both laboratory conditions and in field trials in southern Europe [1] [2]. We are developing HPLC and GCMS-based methods for quantifying the two active diterpenoids (1, 2) and the co-occurring inactive, epimanool (3), to identify the highest yielding Larch sources.

Fig. 1 Structures of larixyl acetate (1), larixol (2) and epimanool (3) present in Larch extracts.

 

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Conflict of Interest

The authors declare no conflict of interest.

• References

• 1 Mulholland DA, Thuerig B, Langat MK, Tamm L, Nawrot DA, James EE, Qayyum M, Shen D, Ennis K, Jones A, Hokkanen H, Demidova N, Izotov D, Schärer H.. Efficacy of extracts from eight economically important forestry species against grapevine downy mildew (Plasmopara viticola) and identification of active constituents. Crop Protection 2017; 102: 104-109
• 2 Thuerig B, James EE, Schärer H, Langat MK, Mulholland DA, Treutwein J, Kleeberg I, Ludwig M, Jayarajah P, Giovannini O, Markelloue E, Tamm L.. Reducing copper use in the environment: the use of larixol and larixyl acetate to treat downy mildew caused by Plasmopara viticola in viticulture. Pest Management Science 2017; 74: 477-488

Publikationsverlauf

Artikel online veröffentlicht:
16. November 2023